Document Detail


CLM94, a Novel Cyclic Amide with Anti-VEGFR-2 and Antiangiogenic Properties, Is Active against Primary Anaplastic Thyroid Cancer in Vitro and in Vivo.
MedLine Citation:
PMID:  22278419     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Context and Objective:We have studied the antitumor activity of a novel cyclic amide, CLM94, with anti-vascular endothelial growth factor (VEGF) receptor-2 and antiangiogenic activity in primary anaplastic thyroid cancer (ATC) cells in vitro and in vivo.Design and Main Outcome Measures:CLM94 was tested: 1) in two human cell lines (HMVEC-d, dermal microvascular endothelial cells; and 8305C, undifferentiated thyroid cancer) at 0.001-100 μm; 2) in ATC cells at the concentrations of 10, 30, and 50 μm; and 3) in an ATC cell line (AF) in CD nu/nu mice.Results:CLM94 significantly inhibited VEGF receptor-2 and epidermal growth factor receptor phosphorylation in HMVEC-d and proliferation in HMVEC-d and 8305C cells. A significant reduction of proliferation with CLM94 in ATC cells (P < 0.01, ANOVA) and a slight but significant reduction of proliferation with CLM94 30 and 50 μm in normal thyroid follicular cells (P < 0.01, ANOVA) were shown. CLM94 increased the percentage of apoptotic ATC cells dose-dependently (P < 0.001, ANOVA) and inhibited migration (P < 0.01) and invasion (P < 0.001). AF cell line was injected sc in CD nu/nu mice, and tumor masses became detectable 25 d afterward. CLM94 (40 mg/kg · d) significantly inhibited tumor growth (starting 10 d after the beginning of treatment). CLM94 significantly decreased the VEGF-A gene expression in the AF cell line and the VEGF-A protein and microvessel density in AF tumor tissues.Conclusions:The antitumor and antiangiogenic activity of a new "cyclic amide" compound, CLM94, is very promising in ATC, opening the way to a future clinical evaluation.
Authors:
Alessandro Antonelli; Guido Bocci; Concettina La Motta; Silvia Martina Ferrari; Poupak Fallahi; Ilaria Ruffilli; Andrea Di Domenicantonio; Anna Fioravanti; Stefania Sartini; Michele Minuto; Simona Piaggi; Alessandro Corti; Greta Alì; Teresa Di Desidero; Piero Berti; Gabriella Fontanini; Romano Danesi; Federico Da Settimo; Paolo Miccoli
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-25
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  -     ISSN:  1945-7197     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Internal Medicine (A.A., S.M.F., P.F., I.R., A.D.D.), Division of Pharmacology (G.B., A.F., T.D.D., R.D.), and Departments of Pharmaceutical Science (C.L.M., S.S., F.D.S.), Surgery (M.M., G.A., P.B., G.F., P.M.), and Experimental Pathology (S.P., A.C.), University of Pisa, School of Medicine, 56100 Pisa, Italy.
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