| CK2-defective Arabidopsis plants exhibit enhanced double-strand-break repair rates and reduced survival after exposure to ionizing radiation. | |
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MedLine Citation:
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PMID: 22487192 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The multifunctional protein kinase CK2 is involved in several aspects of DNA damage response (DDR) in mammals. To gain insight into the role of CK2 in plant genome maintenance we studied the response to genotoxic agents of an Arabidopsis CK2 dominant negative mutant (CK2mut plants). CK2mut plants were hypersensitive to a wide range of genotoxins that produce a variety of DNA lesions. However, they were able to activate DDR after exposure to γ-radiation, as shown by accumulation of phosphorylated histone H2AX and up-regulation of sets of radiomodulated genes. Moreover, functional assays showed that mutant plants could quickly repair the DNA damage produced by genotoxins, and that they exhibited a preferential use of non-conservative mechanisms, which might explain plant lethality. The chromatin of CK2mut plants was more sensitive to digestion with micrococcal nuclease, suggesting compaction changes that agreed with the transcriptional changes detected for a number of genes involved in chromatin structure. Furthermore, CK2mut plants were prone to transcriptional gene silencing release upon genotoxic stress. Our results suggest that CK2 is required in the maintenance and control of genomic stability and chromatin structure in plants, and that this process affects several functions, including DNA damage response and DNA repair. Published 2012. This article is a US Government work and is in the public domain in the USA. |
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Authors:
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Jordi Moreno-Romero; Laia Armengot; M Mar Marquès-Bueno; Anne Britt; M Carmen Martínez |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-9 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: - ISSN: 1365-313X ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Published 2012. This article is a US Government work and is in the public domain in the USA. |
Affiliation:
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Departament de Bioquímica i Biologia Molecular. Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain Department of Plant Biology, University of California, Davis, CA 95616, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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