Document Detail


A CHO-cell mutant with a defect in cytokinesis.
MedLine Citation:
PMID:  1027149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a selection procedure designed to enrich for temperature-sensitive mutant cells blocked in mitosis a CHO-cell mutant was isolated which has a defect in cytokinesis as the basis of its temperature-sensitive phenotype. Cultures of the mutant had an abnormally high percentage (ie. 34%) of polyploid cells at the permissive temperature of 34 degress C and showed further increased frequencies of polyploidy as well as many multinucleated cells at 38.5 degrees 39.5 degrees. When the mutant cells were synchronized in metaphase by Colcemid arrest and then placed into fresh medium at nonpermissive temperature, they did not divide although the completion of mitosis appeared cytologically normal. Ultrastructural examination by electron microscopy of such synchronized cells at telophase revealed no specific defects in cellular components other than failure of development of a normal midbody. The sensitivity of the mutant to cytochalasin B and to Colcemid was the same as for wild-type cells. This mutation behaved as recessive in tetraploid cell hybrids constructed by fusing the mutant with a CHO strain which was wild-type with respect to temperature sensitivty.
Authors:
L H Thompson; P A Lindl
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  2     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1977-07-29     Completed Date:  1977-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  387-400     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division* / drug effects
Cell Line*
Colchicine / pharmacology
Cricetinae
Cytochalasin B / pharmacology
Genes, Recessive
Hybrid Cells
Mutation*
Phenotype
Polyploidy
Temperature
Chemical
Reg. No./Substance:
14930-96-2/Cytochalasin B; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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