| CEACAM-1 negatively regulates G-CSF production by breast tumor associated macrophages that mediate tumor angiogenesis. | |
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MedLine Citation:
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PMID: 23319418 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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CEACAM1, a cell adhesion molecule expressed on epithelial cells and activated immune cells, is down-regulated in many cancers and plays a role in inhibition of inflammation in part by inhibition of G-CSF production by myeloid cells. Since macrophages are associated with a poor prognosis in breast cancer, but play important roles in normal breast, we hypothesized that CEACAM1 down-regulation would lead to tumor promotion under inflammatory conditions. Co-cultures of pro-inflammatory M1 macrophages with CEACAM1 negative MCF7 breast cells produced high levels of G-CSF (10 ng/mL) compared to CEACAM1 transfected MCF7/4S cells (1 ng/mL) or anti-inflammatory M2 macrophage co-cultures (0.5 or 0.1 ng/mL, MCF7 or MCF7/4S, respectively). The expression of CEACAM1 on M1s was much greater than for M2s and was only observed in co-cultures with either MCF7 or MCF7/4S cells. When M1 macrophages were mixed with MCF7 cells and implanted in murine mammary fat pads of NOD/SCID mice, tumor size and blood vessel density were significantly greater than MCF7 or MCF7/4S only tumors which were hardly detected after 8 weeks of growth. In contrast, M1 cells had a much reduced effect on MCF7/4S tumor growth and blood vessel density, indicating that the tumor inhibitory effect of CEACAM1 is most likely related to its anti-inflammatory action on inflammatory macrophages. These results support our previous finding that CEACAM1 inhibits both G-CSF production by myeloid cells and G-CSF stimulated tumor angiogenesis. © 2013 Wiley Periodicals, Inc. |
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Authors:
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Sridhar Samieni; Zhifang Zhang; John E Shively |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-15 |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: - ISSN: 1097-0215 ISO Abbreviation: Int. J. Cancer Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2013 UICC. |
Affiliation:
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City of Hope Irell&Manella Graduate School of Biological Sciences, Duarte, CA 91010; Department of Immunology, Beckman Research Institute of City of Hope, 1450 E. Duarte Rd, Duarte CA 91010, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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