| CE Analysis of γ-Globulin Mobility and Potential Clinical Utility. | |
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MedLine Citation:
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PMID: 22976106 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the γ-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins were purified from serum using protein L-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the γ-globulins (r = 0.2-0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test. |
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Authors:
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Dieter Vanderschaeghe; Evi Debruyne; Hans Van Vlierberghe; Nico Callewaert; Joris Delanghe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 919 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 249-57 Citation Subset: IM |
Affiliation:
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Department for Molecular Biomedical Research, Unit for Molecular Glycobiology, VIB, Ghent, Belgium. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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