Document Detail

The CDTA-soluble pectic substances from soybean meal are composed of rhamnogalacturonan and xylogalacturonan but not homogalacturonan.
MedLine Citation:
PMID:  11169388     Owner:  NLM     Status:  MEDLINE    
Structural characteristics of pectic substances extracted from soybean meal cell walls (water unextractable solids) with a chelating agent-containing buffer (0.05M 1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and 0.05M NH(4)-oxalate in 0.05M NaOAc buffer) were studied. The arabinogalactans present as side chains to the rhamnogalacturonan backbone were largely removed by enzymatic hydrolysis using endo-galactanase, exo-galactanase, endo-arabinanase, and arabinofuranosidase B. The remaining pectic backbone appeared to be resistant to enzymatic degradation by pectolytic enzymes. After partial acid hydrolysis of the isolated pectic backbone, one oligomeric and two polymeric populations were obtained by size-exclusion chromatography. Monosaccharide and linkage analyses, enzymatic degradation, and NMR spectroscopy of these populations showed that the pectic substances in the original extract contain both rhamnogalacturonan and xylogalacturonan regions, while homogalacturonan is absent.
M M Huisman; C T Fransen; J P Kamerling; J F Vliegenthart; H A Schols; A G Voragen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biopolymers     Volume:  58     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  279-94     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Inc.
Department of Agrotechnology and Food Sciences, Wageningen University, The Netherlands.
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MeSH Terms
Acids / chemistry
Edetic Acid / analogs & derivatives*,  chemistry
Enzymes / chemistry
Magnetic Resonance Spectroscopy
Molecular Conformation
Pectins / chemistry*
Soybeans / chemistry*
Reg. No./Substance:
0/Acids; 0/Enzymes; 0/Pectins; 482-54-2/CDTA; 60-00-4/Edetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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