| CDKN1C (p57KIP2) mRNA expression in human retinoblastomas. | |
| | |
MedLine Citation:
|
PMID: 20565244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: Quantify cyclin dependent kinase inhibitor 1C (CDKN1C or p57KIP2) mRNA levels in human retinoblastoma tumors (RB) and associate with disease phenotype. METHODS: CDKN1C mRNA expression was quantified in 55 RB, 3 retinoblastoma cell lines, and 12 control retinas by real time PCR. Localization of CDKN1C protein was confirmed by immunohistochemistry. Tumor CDKN1C expression levels were correlated with phenotype. RESULTS: Fifty-three of 55 tumors showed significantly elevated levels of CDKN1C mRNA relative to age-matched or adult retina controls. No significant difference was seen relative to fetal retinal controls or retinoblastoma cell lines. Immunohistochemistry revealed heterogeneous staining of CDKN1C protein in tumor cells, which was mainly nuclear although some protein appeared to be cytoplasmic. No correlation was observed between the CDKN1C mRNA expression levels and tumor phenotype. CONCLUSION: High levels of CDKN1C expression are common in RB. It remains to be determined if elevated expression is a protective response to transformation, provides a selective advantage to tumor cells, or simply reflects the presence of dividing cells. |
| | |
Authors:
|
Jagadeesan Madhavan; Kandalam Mallikarjuna; Khetan Vikas; Ronnie George; Rod Bremner; Govindasamy Kumaramanickavel |
Related Documents
:
|
18930834 - Diurnal expressions of four subtypes of melatonin receptor genes in the optic tectum an... 17341594 - An intron control region differentially regulates expression of thyroid hormone recepto... 12565814 - Cralbp transcriptional regulation in ciliary epithelial, retinal müller and retinal pi... 19059364 - Expression patterns of the tmem16 gene family during cephalic development in the mouse. 20008534 - Klebsiella pneumoniae capsule polysaccharide impedes the expression of beta-defensins b... 17849424 - Inducible nitric oxide synthase, nitrotyrosine and p53 mutations in the molecular patho... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Ophthalmic genetics Volume: 31 ISSN: 1744-5094 ISO Abbreviation: Ophthalmic Genet. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-06 Completed Date: 2010-10-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9436057 Medline TA: Ophthalmic Genet Country: England |
Other Details:
|
Languages: eng Pagination: 141-6 Citation Subset: IM |
Affiliation:
|
SN ONGC Department of Genetics & Molecular Biology, Vision Research Foundation, Sankara Nethralaya, Tamil Nadu, India. jmadhavan2002@yahoo.co.in |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cyclin-Dependent Kinase Inhibitor p57 / genetics*, metabolism Fetus Gestational Age Humans Immunoenzyme Techniques Infant Middle Aged Phenotype RNA, Messenger / genetics* Retinal Neoplasms / genetics*, metabolism Retinoblastoma / genetics*, metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/CDKN1C protein, human; 0/Cyclin-Dependent Kinase Inhibitor p57; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In Process Citation
Next Document: Familial case of Blau syndrome associated with a CARD15/NOD2 mutation.