Document Detail


The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.
MedLine Citation:
PMID:  15549137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G1 phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb-CDK activity.
Authors:
Yael Aylon; Batia Liefshitz; Martin Kupiec
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-18
Journal Detail:
Title:  The EMBO journal     Volume:  23     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-08     Completed Date:  2005-03-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  4868-75     Citation Subset:  IM    
Affiliation:
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cyclin-Dependent Kinases / genetics,  metabolism*
DNA Damage*
DNA Repair*
Recombination, Genetic*
Saccharomyces cerevisiae / genetics,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; EC 2.7.11.22/Cyclin-Dependent Kinases
Comments/Corrections

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