Document Detail


CD99 isoforms expression dictates T cell functional outcomes.
MedLine Citation:
PMID:  12368226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CD99, a unique integral membrane protein present on the surface of all human T cells, has previously been shown to regulate cell function and fate. In peripheral T cells, it triggers immediate activation of alpha4b1 integrin and cell arrest on inflamed vascular endothelium, whereas it mediates an apoptotic signal in double-positive thymocytes undergoing the selection process. Two isoforms of CD99 exist, a long form corresponding to the full-length protein and a short form harboring a deletion in the intracytoplasmic segment. Here, we show that while peripheral T cells display exclusive expression of the long form, double-positive thymocytes express both isoforms. Moreover, differential expression of these two CD99 molecules can lead to distinct functional outcomes. Expression of the long form in a CD99-deficient Jurkat T cell line is sufficient to promote CD99-induced cell adhesion, whereas coexpression of the two isoforms is required to trigger T-cell death. When coexpressed, the two proteins form covalent heterodimers, which locate within glycosphingolipidic rafts and induce sphingomyelin degradation. Cholesterol depletion experiments show that this localization is required for the induction of apoptosis. Thus, the surface expression pattern of CD99 isoforms determines T-cell functional outcomes.
Authors:
Isabelle Alberti; Ghislaine Bernard; Alexandre K Rouquette-Jazdanian; Claudette Pelassy; Monique Pourtein; Claude Aussel; Alain Bernard
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Publication Detail:
Type:  Journal Article     Date:  2002-10-04
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  16     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-06     Completed Date:  2002-12-19     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1946-8     Citation Subset:  IM    
Affiliation:
INSERM U343 et Laboratoire d'Immunologie, Université de Nice-Sophia Antipolis, France.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis,  metabolism*,  physiology*
Apoptosis
Cell Adhesion
Cell Adhesion Molecules / analysis,  metabolism*,  physiology*
Cell Differentiation
Dimerization
Humans
Jurkat Cells
Membrane Microdomains / chemistry
Protein Isoforms / metabolism,  physiology
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/CD99 protein, human; 0/Cell Adhesion Molecules; 0/Protein Isoforms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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