| CD95 and TNFα-induced apoptosis in liver metastases of colorectal carcinoma. | |
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MedLine Citation:
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PMID: 20952729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Therapeutic options in patients with metastatic colorectal cancer are still limited. As apoptosis contributes to the overall sensitivity to radiotherapy or chemotherapy, a better understanding of the apoptotic process in metastatic tumour tissues is necessary. MATERIALS AND METHODS: Precision cut tissue slices (PCTS) of three human liver metastases were used to investigate the effect of activating CD95 antibodies (concentrations: 0.1 μg/ml, 1 μg/ml and 1 μg/ml and 1 μg/ml actinomycin D) as well as TNFα (concentrations 1 ng/ml; 10 ng/ml and 10 ng/ml and 1 μg/ml actinomycin D) directly in tumour tissue after 6 h, 12 h and 24 h. The apoptotic effect was assessed immunohistochemically. RESULTS: Activating CD95 antibodies combined with actino-mycin D led to a significant increase in apoptosis after 12 h. Using TNFα at a high dosage, a significant increase in the apoptosis rate was observed after 6 h and after 12 h in all dosage groups. CONCLUSIONS: PCTS can be used to investigate the effect of different apoptotic signals directly in human tumour tissues. TNFα is able to effectively induce apoptosis in liver metastases of colorectal carcinoma. Thus, the extrinsic pathway of apoptosis may be a promising target in the development of new therapeutic approaches. |
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Authors:
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Hans-Udo Kasper; Elisabeth Konze; Michael Kern; Dirk Ludger Stippel |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: In vivo (Athens, Greece) Volume: 24 ISSN: 1791-7549 ISO Abbreviation: In Vivo Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806809 Medline TA: In Vivo Country: Greece |
Other Details:
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Languages: eng Pagination: 653-7 Citation Subset: IM |
Affiliation:
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Institut für Pathologie, Medical Center am Clemenshospital, Düesberweg 128, D-48153 Münster, Germany. email@patho-muenster.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Antibiotics, Antineoplastic / pharmacology Antibodies / pharmacology Antigens, CD95 / immunology, metabolism* Apoptosis / drug effects*, physiology Colorectal Neoplasms / pathology* Dactinomycin / pharmacology Drug Discovery / methods Female Humans Liver Neoplasms / drug therapy*, secondary* Male Microtomy / methods Signal Transduction / drug effects, physiology Tumor Necrosis Factor-alpha / immunology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Antibodies; 0/Antigens, CD95; 0/FAS protein, human; 0/Tumor Necrosis Factor-alpha; 50-76-0/Dactinomycin |
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