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CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures.
MedLine Citation:
PMID:  19296231     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cluster Differentiation 90 (CD90) is a cell surface glycoprotein originally identified on mouse thymocytes. Although CD90 has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells, the knowledge about the levels of CD90 expression on different cell types, including human primary cells, is limited. The goal of this study was to identify CD90 as a human primary cell biomarker and to develop an efficient and reliable method for eliminating unwanted or contaminating fibroblasts from human primary cell cultures suitable for research pursuant to cell based therapy technologies.
Authors:
Lynn Kisselbach; Michael Merges; Alexis Bossie; Ann Boyd
Publication Detail:
Type:  Journal Article     Date:  2009-03-19
Journal Detail:
Title:  Cytotechnology     Volume:  59     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-05-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  31-44     Citation Subset:  B    
Affiliation:
Department of Cell Therapy, Lonza Walkersville, Inc., Walkersville, MD, 21793, USA, lynn.kisselbach@lonza.com.
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