Document Detail


CD9 clustering and formation of microvilli zippers between contacting cells regulates virus-induced cell fusion.
MedLine Citation:
PMID:  18363777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Members of the tetraspanin family including CD9 contribute to the structural organization and plasticity of the plasma membrane. K41, a CD9-specific monoclonal antibody, inhibits the release of HIV-1 and canine distemper virus (CDV)- but not measles virus (MV)-induced cell-cell fusion. We now report that K41, which recognizes a conformational epitope on the large extracellular loop of CD9, induces rapid relocation and clustering of CD9 in net-like structures at cell-cell contact areas. High-resolution analyses revealed that CD9 clustering is accompanied by the formation of microvilli that protrude from either side of adjacent cell surfaces, thus forming structures like microvilli zippers. While the cellular CD9-associated proteins beta(1)-integrin and EWI-F were co-clustered with CD9 at cell-cell interfaces, viral proteins in infected cells were differentially affected. MV envelope proteins were detected within CD9 clusters, whereas CDV proteins were excluded from CD9 clusters. Thus, the tetraspanin CD9 can regulate cell-cell fusion by controlling the access of the fusion machinery to cell contact areas.
Authors:
Katrin Singethan; Nora Müller; Sabine Schubert; Doreen Lüttge; Dimitry N Krementsov; Sandhya R Khurana; Georg Krohne; Sibylle Schneider-Schaulies; Markus Thali; Jürgen Schneider-Schaulies
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-03-17
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  9     ISSN:  1600-0854     ISO Abbreviation:  Traffic     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-09     Completed Date:  2008-07-16     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  924-35     Citation Subset:  IM    
Affiliation:
Institute for Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, D-97078 Würzburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology,  metabolism,  ultrastructure
Antibodies, Viral / immunology,  ultrastructure
Antigens, CD / immunology*,  metabolism,  ultrastructure
Antigens, CD9
CHO Cells
Cell Communication
Cell Fusion*
Cells, Cultured
Cercopithecus aethiops
Cricetinae
Cricetulus
Distemper Virus, Canine / genetics,  pathogenicity*
Dogs
Endothelial Cells / immunology,  ultrastructure,  virology
Endothelium, Vascular / cytology
Fluorescent Antibody Technique, Indirect
HeLa Cells
Humans
Kinetics
Measles virus / genetics,  pathogenicity*
Membrane Glycoproteins / immunology*,  metabolism,  ultrastructure
Microvilli / metabolism*,  ultrastructure
Transfection
Umbilical Veins / cytology
Vero Cells
Grant Support
ID/Acronym/Agency:
R01 AI080302/AI/NIAID NIH HHS; R01 AI080302-01A1S1/AI/NIAID NIH HHS; R56 AI047727/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Antigens, CD; 0/Antigens, CD9; 0/CD9 protein, human; 0/Membrane Glycoproteins
Comments/Corrections

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