| CD9 Is Expressed on Human Male Germ Cells That Have a Long-Term Repopulation Potential after Transplantation into Mouse Testes. | |
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MedLine Citation:
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PMID: 22592495 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Human spermatogonial stem cells (SSCs) play critical roles in life-long maintenance of male fertility and regeneration of spermatogenesis. These cells are expected to provide an important resource for male fertility preservation and restoration. A basic strategy has been proposed to involve harvesting testis biopsy specimens from a cancer patient prior to cancer therapies, and transplanting back to the patient at a later time; then, SSCs included in the specimens regenerate spermatogenesis. To clinically apply this strategy, isolating live human SSCs is important. In this study, we investigated if CD9, a known rodent SSC marker, is expressed on human male germ cells that can repopulate recipient mouse testes upon transplantation. Testicular tissues were obtained from men with obstructive azoospermia. Using immunohistochemistry, we found that CD9 was expressed in human male germ cells in the basal compartment of the seminiferous epithelium. Following immunomagnetic cell sorting, CD9-positive cells were enriched for germ cells expressing MAGEA4, which is expressed by spermatogonia and some early spermatocytes, compared to unsorted cells. We then transplanted CD9-positive cells into nude mouse testes and detected a ~3- to 4-fold enrichment of human germ cells that repopulated mouse testes for at least four months after transplantation, compared to unsorted cells. We also observed that some cell turnover occurred in human germ cell colonies in recipient testes. These results demonstrate that CD9 identifies human male germ cells with capability of long-term survival and cell turnover in the xenogeneic testis environment. |
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Authors:
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Khaled Zohni; Xiangfan Zhang; Seang Lin Tan; Peter Chan; Makoto Nagano |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-16 |
Journal Detail:
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Title: Biology of reproduction Volume: - ISSN: 1529-7268 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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