| CD80 and CD86 Differentially Regulate Mechanical Interactions of T-Cells with Antigen-Presenting Dendritic Cells and B-Cells. | |
| | |
MedLine Citation:
|
PMID: 23024807 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Functional T-cell responses are initiated by physical interactions between T-cells and antigen-presenting cells (APCs), including dendritic cells (DCs) and B-cells. T-cells are activated more effectively by DCs than by B-cells, but little is known about the key molecular mechanisms that underpin the particular potency of DC in triggering T-cell responses. To better understand the influence of physical intercellular interactions on APC efficacy in activating T-cells, we used single cell force spectroscopy to characterize and compare the mechanical forces of interactions between DC:T-cells and B:T-cells. Following antigen stimulation, intercellular interactions of DC:T-cell conjugates were stronger than B:T-cell interactions. DCs induced higher levels of T-cell calcium mobilization and production of IL-2 and IFNγ than were elicited by B-cells, thus suggesting that tight intercellular contacts are important in providing mechanically stable environment to initiate T-cell activation. Blocking antibodies targeting surface co-stimulatory molecules CD80 or CD86 weakened intercellular interactions and dampen T-cell activation, highlighting the amplificatory roles of CD80/86 in regulating APC:T-cell interactions and T-cell functional activation. The variable strength of mechanical forces between DC:T-cells and B:T-cell interactions were not solely dependent on differential APC expression of CD80/86, since DCs were superior to B-cells in promoting strong interactions with T-cells even when CD80 and CD86 were inhibited. These data provide mechanical insights into the effects of co-stimulatory molecules in regulating APC:T-cell interactions. |
| | |
Authors:
|
Tong Seng Lim; James Kang Hao Goh; Alessandra Mortellaro; Chwee Teck Lim; Günter J Hämmerling; Paola Ricciardi-Castagnoli |
Related Documents
:
|
23404367 - Antiproliferative activity of alpinia officinarum extract in the human breast cancer ce... 23494127 - Biochemistry, proteomics, and phosphoproteomics of plant mitochondria from non-photosyn... 23027217 - Current concepts in the etiology and treatment of keloids. 23554077 - Interstitial engraftment of adipose-derived stem cells into an acellular dermal matrix ... 16557947 - Effect of ca on the synthesis of deoxyribonucleic acid in virulent and avirulent yersinia. 6354437 - Enhancement of doxorubicin and vinblastine sensitivity in anthracycline-resistant p388 ... |
Publication Detail:
|
Type: Journal Article Date: 2012-09-14 |
Journal Detail:
|
Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-10-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
|
Languages: eng Pagination: e45185 Citation Subset: IM |
Affiliation:
|
Singapore Immunology Network, Agency for Science, Technology and Research (ASTAR), Singapore, Singapore. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IP-10 can be measured in dried plasma spots in patients with chronic hepatitis C infection.
Next Document: Functional specialization in proline biosynthesis of melanoma.