| CD8αα and -αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I. | |
| | |
MedLine Citation:
|
PMID: 22081144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bimolecular fluorescence complementation was used to engineer CD8 molecules so that CD8αα and CD8αβ dimers can be independently visualized on the surface of a T cell during antigen recognition. Using this approach, we show that CD8αα is recruited to the immunological synapse almost as well as CD8αβ, but because the kinase Lck associates preferentially with CD8αβ in lipid rafts, CD8αα is the weaker co-receptor. During recognition of the strong CD8αα ligand H2-TL, CD8αα is preferentially recruited. Thus, recruitment of the two CD8 species correlates with their relative binding to the available ligands, rather than with the co-receptor functions of the CD8 species. |
| | |
Authors:
|
Vasily Rybakin; Jean-Pierre Clamme; Jeanette Ampudia; Pia P Yachi; Nicholas R J Gascoigne |
Related Documents
:
|
9618464 - Analysis of human cytochrome p450 3a4 cooperativity: construction and characterization ... 11377984 - Site-directed mutagenesis studies of rat steroid 5alpha-reductase (isozyme-1): mutation... 7372654 - Substrate stabilization of lysozyme to thermal and guanidine hydrochloride denaturation. 10623614 - Effect of electrostatic interactions on the binding of charged substrate to groel studi... 8420804 - Conformational changes in subdomain-2 of g-actin upon polymerization into f-actin and u... 14516204 - Ablation of the liver fatty acid binding protein gene decreases fatty acyl coa binding ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-12-01 |
Journal Detail:
|
Title: EMBO reports Volume: 12 ISSN: 1469-3178 ISO Abbreviation: EMBO Rep. Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-01 Completed Date: 2012-03-21 Revised Date: 2013-02-19 |
Medline Journal Info:
|
Nlm Unique ID: 100963049 Medline TA: EMBO Rep Country: England |
Other Details:
|
Languages: eng Pagination: 1251-6 Citation Subset: IM |
Affiliation:
|
Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037-1092, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD8 / metabolism* Fluorescence Histocompatibility Antigens Class I / immunology* Immunological Synapses / immunology* Ligands Membrane Glycoproteins / immunology Mice Protein Binding Protein Isoforms / metabolism Receptors, Antigen, T-Cell / immunology Recombinant Fusion Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
R01AI074074/AI/NIAID NIH HHS; R01GM065230/GM/NIGMS NIH HHS; T32HL07195/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD8; 0/CD8 antigen, alpha chain; 0/CD8alphabeta antigen; 0/Histocompatibility Antigens Class I; 0/Ligands; 0/Membrane Glycoproteins; 0/Protein Isoforms; 0/Receptors, Antigen, T-Cell; 0/Recombinant Fusion Proteins; 0/thymus-leukemia antigens |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Superinfection in malaria: Plasmodium shows its iron will.
Next Document: Dual-isotope SPECT imaging of striatal dopamine: a comparative study between never-treated and halop...