Document Detail


CD8αα and -αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I.
MedLine Citation:
PMID:  22081144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bimolecular fluorescence complementation was used to engineer CD8 molecules so that CD8αα and CD8αβ dimers can be independently visualized on the surface of a T cell during antigen recognition. Using this approach, we show that CD8αα is recruited to the immunological synapse almost as well as CD8αβ, but because the kinase Lck associates preferentially with CD8αβ in lipid rafts, CD8αα is the weaker co-receptor. During recognition of the strong CD8αα ligand H2-TL, CD8αα is preferentially recruited. Thus, recruitment of the two CD8 species correlates with their relative binding to the available ligands, rather than with the co-receptor functions of the CD8 species.
Authors:
Vasily Rybakin; Jean-Pierre Clamme; Jeanette Ampudia; Pia P Yachi; Nicholas R J Gascoigne
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-01
Journal Detail:
Title:  EMBO reports     Volume:  12     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-01     Completed Date:  2012-03-21     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  1251-6     Citation Subset:  IM    
Affiliation:
Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037-1092, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD8 / metabolism*
Fluorescence
Histocompatibility Antigens Class I / immunology*
Immunological Synapses / immunology*
Ligands
Membrane Glycoproteins / immunology
Mice
Protein Binding
Protein Isoforms / metabolism
Receptors, Antigen, T-Cell / immunology
Recombinant Fusion Proteins / metabolism
Grant Support
ID/Acronym/Agency:
R01AI074074/AI/NIAID NIH HHS; R01GM065230/GM/NIGMS NIH HHS; T32HL07195/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD8; 0/CD8 antigen, alpha chain; 0/CD8alphabeta antigen; 0/Histocompatibility Antigens Class I; 0/Ligands; 0/Membrane Glycoproteins; 0/Protein Isoforms; 0/Receptors, Antigen, T-Cell; 0/Recombinant Fusion Proteins; 0/thymus-leukemia antigens
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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