Document Detail

CD73, a novel cell surface antigen that characterizes retinal photoreceptor precursor cells.
MedLine Citation:
PMID:  19515998     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The authors sought to identify cell surface markers of photoreceptor and its precursor cells. METHODS: The expression of surface CD antigens that label both temporally and spatially distinct populations of mouse retinal cells were examined. Of the antibodies that showed positive signals in retinal cells, CD73 was focused on for more detailed analyses. RESULTS: Mouse retinal subpopulations that expressed CD73 first appeared around birth and subsequently increased dramatically in number, eventually representing more than 90% of the retinal cells in the adult. CD73(+) cells were postmitotic and mostly rhodopsin-negative at postnatal day 1. However, in the adult retina, most of these cells expressed rhodopsin but not s-opsin. In reaggregation cultures, CD73(+) cells differentiated into rhodopsin-positive cells more rapidly than CD73(-) cells, which supports the idea that CD73 is an early photoreceptor lineage marker. The effects of ectopic expression in retinal cells of Nrl and Crx, both of which are transcription factors known to be expressed in photoreceptor lineage, suggest that CD73 is genetically downstream of Crx in the rod cell differentiation lineage. Adult retina of the common marmoset monkey also showed correlation of the expression pattern of rhodopsin and CD73. CONCLUSIONS: CD73 is a cell surface marker of cone/rod common precursors and mature rod cells in mice and is genetically localized between Nrl and Crx. The expression of CD73 was conserved in primate rod cells, and CD73 provides an useful tool to purify photoreceptor cells for transplantation aimed at the regeneration of photoreceptors.
Hideto Koso; Chiharu Minami; Yoko Tabata; Mariko Inoue; Erika Sasaki; Shinya Satoh; Sumiko Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-10
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  50     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-03     Completed Date:  2009-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5411-8     Citation Subset:  IM    
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
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MeSH Terms
5'-Nucleotidase / genetics,  metabolism*
Antigens, Surface / metabolism*
Basic-Leucine Zipper Transcription Factors / genetics
Biological Markers / metabolism*
Cell Differentiation / physiology
Cell Lineage*
Cell Proliferation
Eye Proteins / genetics
Flow Cytometry
Gene Expression
Homeodomain Proteins / genetics
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Transgenic
Photoreceptor Cells, Vertebrate / cytology,  metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Rhodopsin / metabolism
Stem Cells / cytology,  metabolism*
Trans-Activators / genetics
Reg. No./Substance:
0/Antigens, Surface; 0/Basic-Leucine Zipper Transcription Factors; 0/Biological Markers; 0/Eye Proteins; 0/Homeodomain Proteins; 0/Nrl protein, mouse; 0/RNA, Messenger; 0/Trans-Activators; 0/cone rod homeobox protein; 9009-81-8/Rhodopsin; EC'-Nucleotidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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