| CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells. | |
| | |
MedLine Citation:
|
PMID: 21412255 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
In this study, we have investigated the role of CD69, an early inducible leukocyte activation receptor, in murine dendritic cell (DC) differentiation, maturation, and migration. Skin DCs and DC subsets present in mouse lymphoid organs express CD69 in response to maturation stimuli. Using a contact sensitization model, we show that skin DCs migrated more efficiently to draining lymph nodes (LNs) in the absence of CD69. This was confirmed by subcutaneous transfer of CD69-/- DCs, which presented an increased migration to peripheral LNs. Two-photon microscopy analysis showed that once DCs reached the LNs, CD69 deficiency did not alter DC interstitial motility in the LNs. Chemotaxis to sphingosine-1-phosphate (S1P) was enhanced in CD69-/- DCs compared with wild-type DCs. Accordingly, we detected a higher expression of S1P receptor type-1 (S1P(1)) by CD69-/- DCs, whereas S1P(3) expression levels were similar in wild-type and CD69-/- DCs. Moreover, in vivo treatment with S1P analogs SEW2871 and FTY720 during skin sensitization reduced skin DC migration to peripheral LNs. These results suggest that CD69 regulates S1P-induced skin DC migration by modulating S1P(1) function. Together, our findings increase our knowledge on DC trafficking patterns in the skin, enabling the development of new directed therapies using DCs for antigen (Ag) delivery.Journal of Investigative Dermatology advance online publication, 17 March 2011; doi:10.1038/jid.2011.54. |
| | |
Authors:
|
Amalia Lamana; Pilar Martin; Hortensia de la Fuente; Laura Martinez-Muñoz; Aranzazu Cruz-Adalia; Marta Ramirez-Huesca; Cristina Escribano; Kathrin Gollmer; Mario Mellado; Jens V Stein; Jose Luis Rodriguez-Fernandez; Francisco Sanchez-Madrid; Gloria Martinez Del Hoyo |
Related Documents
:
|
22092535 - Molecular and clinical rationale for therapeutic targeting of interleukin-5 and its rec... 14744135 - Apoptosis of insulin-secreting cells induced by endoplasmic reticulum stress is amplifi... 12480495 - Stress and the inflammatory response: a review of neurogenic inflammation. 8771625 - Psychosocial and immune effects of self-hypnosis training for stress management through... 11111925 - Immune modulation by the cholera-like enterotoxin b-subunits: from adjuvant to immunoth... 22092535 - Molecular and clinical rationale for therapeutic targeting of interleukin-5 and its rec... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-3-17 |
Journal Detail:
|
Title: The Journal of investigative dermatology Volume: - ISSN: 1523-1747 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-3-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Servicio de Inmunología, Hospital de la Princesa, Universidad Autónoma de Madrid, Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Testicular germ cell tumours: predisposition genes and the male germ cell niche.
Next Document: cis-Urocanic Acid Enhances Prostaglandin E(2) Release and Apoptotic Cell Death via Reactive Oxygen S...