| CD45RO enriches for activated, highly mutated human germinal center B cells. | |
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MedLine Citation:
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PMID: 17644737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To date, there is no consensus regarding the influence of different CD45 isoforms during peripheral B-cell development. Examining correlations between surface CD45RO expression and various physiologic processes ongoing during the germinal center (GC) reaction, we hypothesized that GC B cells, like T cells, that up-regulate surface RO should progressively acquire phenotypes commonly associated with activated, differentiating lymphocytes. GC B cells (IgD(-)CD38(+)) were subdivided into 3 surface CD45RO fractions: RO(-), RO(+/-), and RO(+). We show here that the average number of mutations per IgV(H) transcript increased in direct correlation with surface RO levels. Conjunctional use of RO and CD69 further delineated low/moderately and highly mutated fractions. Activation-induced cytidine deaminase (AID) mRNA was slightly reduced among RO(+) GC B cells, suggesting that higher mutation averages are unlikely due to elevated somatic mutation activity. Instead, RO(+) GC B cells were negative for Annexin V, comprised mostly (93%) of CD77(-) centrocytes, and were enriched for CD69(+) cells. Collectively, RO(+) GC B cells occupy what seems to be a specialized niche comprised mostly of centrocytes that may be in transition between activation states. These findings are among the first to sort GC B cells into populations enriched for live mutated cells solely using a single extracellular marker. |
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Authors:
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Stephen M Jackson; Natessa Harp; Darshna Patel; Jeffrey Zhang; Savannah Willson; Yoon J Kim; Christian Clanton; J Donald Capra |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-07-20 |
Journal Detail:
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Title: Blood Volume: 110 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-20 Completed Date: 2007-12-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 3917-25 Citation Subset: AIM; IM |
Affiliation:
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Molecular Immunogenetics Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD / immunology, metabolism Antigens, CD45* B-Lymphocytes / immunology*, metabolism Child Child, Preschool Cytidine Deaminase / immunology, metabolism Female Germinal Center / cytology, immunology*, metabolism Humans Immunoglobulin D Immunoglobulin Heavy Chains / genetics, immunology*, metabolism Immunoglobulin Variable Region / genetics, immunology*, metabolism Infant Lymphocyte Activation / immunology* Male RNA, Messenger / immunology, metabolism Somatic Hypermutation, Immunoglobulin / immunology* T-Lymphocytes / cytology, immunology, metabolism Up-Regulation / immunology |
| Grant Support | |
ID/Acronym/Agency:
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AI 12127/AI/NIAID NIH HHS; P20RR15577/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Immunoglobulin D; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region; 0/RNA, Messenger; EC 3.1.3.48/Antigens, CD45; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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