Document Detail

CD44H plays an important role in peritoneal dissemination of scirrhous gastric cancer cells.
MedLine Citation:
PMID:  9045958     Owner:  NLM     Status:  MEDLINE    
The role of the adhesion molecule CD44H in the peritoneal adhesion and invasion of cancer cells was assessed using cell lines with low and high peritoneal seeding ability, OCUM-2M (2M) and OCUM-2MD3 (2MD3), respectively. The in vitro binding ability to peritoneal components (mesothelial cells, fibronectin and type I collagen) and invasive ability of 2MD3 cells were higher than those of 2M cells. The expression level of CD44H on 2MD3 cells was higher than that on 2M cells as determined by western blot analysis and flow cytometry. The adhesiveness of 2MD3 cells to hyaluronic acid, which is expressed on the surfaces of mesothelial cells, was greater than that of 2M cells. The binding ability of 2MD3 cells to mesothelial cells was inhibited in the presence of anti-CD44H monoclonal antibody, but that of 2M cells was not. These results suggested that the 2MD3 cell binding to mesothelial cells is regulated by the CD44-hyaluronic acid dependent system. The in vitro binding to submesothelial components and the invasiveness of 2MD3 cells were also inhibited in the presence of anti-CD44H antiody. The in vivo inoculation of 2MD3 cells treated with an anti-CD44H antibody resulted in a significiant prolongation of survival time as compared with control mice that were inoculated with 2MD3 cells alone. In conclusion, CD44H was associated with attachment not only to hyaluronic acid on mesothelial cells, but also to peritoneal stromal components. Thus, CD44H may play an important role in cancer cell binding and invasion in the peritoneal dissemination of scirrhous gastric cancer cells.
S Nishimura; Y S Chung; M Yashiro; T Inoue; M Sowa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  87     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-03-25     Completed Date:  1997-03-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1235-44     Citation Subset:  IM    
First Department of Surgery, Osaka City University Medical School, Abeno-ku.
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MeSH Terms
Adenocarcinoma, Scirrhous / chemistry,  pathology*
Antibodies / metabolism
Antigens, CD44 / analysis,  physiology*
Blotting, Western
Cell Adhesion
Epithelium / metabolism
Extracellular Matrix / metabolism
Neoplasm Invasiveness
Peritoneum / pathology*
Stomach Neoplasms / chemistry,  pathology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD44

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