| CD44 is expressed in non-myelinating Schwann cells of the adult rat, and may play a role in neurodegeneration-induced glial plasticity at the neuromuscular junction. | |
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MedLine Citation:
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PMID: 19385056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CD44 is a multifunctional cell surface glycoprotein which regulates cell-cell and cell-matrix interactions in a variety of tissues. In particular, the protein was found to be expressed in glial cells of developing, but not adult, peripheral nerves, where it takes part in signaling mediated by ErbB class of receptors for neuregulins. Here, we demonstrate, using high resolution morphological methods, tissue fractionation and RT-PCR, that CD44 is strongly expressed in terminal Schwann cell (TSC) at the neuromuscular junction (NMJ) of the adult rat skeletal muscle. As CD44 is also expressed by Schwann cells of the non-myelinated Remak bundles of the proximal peripheral nerves, it appears to be a marker of non-myelinating Schwann cell subpopulation. The analysis of transgenic rats bearing a mutated superoxide-dismutase gene (SOD1(G93A)) causing familial amyotrophic lateral sclerosis (ALS) revealed that TSC activation and morphological plasticity at the NMJ, caused by ongoing denervation-reinnervation is associated with a strong increase in CD44 expression therein. Notably, CD44 immunoreactivity is present in fine axon-escheating processes of the glial cells that guide reinnervation. In addition, we found that both in normal and SOD1(G93A) muscle, CD44 expressed in TSC partially colocalizes with immunoreactivities of neuregulin receptors ErbB2 and ErbB3. The colocalization appears to reflect a physical interaction, as evidenced by co-immunoprecipitation and fluorescence resonance energy transfer (FRET) analysis between CD44 and ErbB3. Importantly, TSC activation upon ALS-like neurodegeneration results in significant increase in molecular proximity of CD44 and ErbB3, which may have an impact on glial plasticity at the NMJ. |
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Authors:
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Adam Gorlewicz; Jakub Wlodarczyk; Ewa Wilczek; Maciej Gawlak; Anna Cabaj; Henryk Majczynski; Klaudia Nestorowicz; Magdalena Aneta Herbik; Pawel Grieb; Urszula Slawinska; Leszek Kaczmarek; Grzegorz M Wilczynski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurobiology of disease Volume: 34 ISSN: 1095-953X ISO Abbreviation: Neurobiol. Dis. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-21 Completed Date: 2009-08-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9500169 Medline TA: Neurobiol Dis Country: United States |
Other Details:
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Languages: eng Pagination: 245-58 Citation Subset: IM |
Affiliation:
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Nencki Institute of Experimental Biology, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Aging / metabolism Amyotrophic Lateral Sclerosis / immunology, metabolism, physiopathology Animals Antigens, CD44 / genetics, metabolism* Fluorescence Resonance Energy Transfer Glycoproteins / analysis, metabolism Male Nerve Degeneration / immunology, metabolism*, physiopathology Nerve Fibers, Unmyelinated / metabolism, ultrastructure Neuroglia / cytology, metabolism* Neuromuscular Junction / immunology, metabolism*, physiopathology Neuronal Plasticity / physiology* RNA, Messenger / analysis, metabolism Rats Rats, Sprague-Dawley Rats, Transgenic Receptor, erbB-3 / analysis, metabolism Schwann Cells / cytology, metabolism* Superoxide Dismutase / genetics |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD44; 0/Erbb2 protein, rat; 0/Glycoproteins; 0/RNA, Messenger; EC 1.15.1.-/superoxide dismutase 1; EC 1.15.1.1/Superoxide Dismutase; EC 2.7.10.1/Receptor, erbB-3 |
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