Document Detail

CD44 is expressed in non-myelinating Schwann cells of the adult rat, and may play a role in neurodegeneration-induced glial plasticity at the neuromuscular junction.
MedLine Citation:
PMID:  19385056     Owner:  NLM     Status:  MEDLINE    
CD44 is a multifunctional cell surface glycoprotein which regulates cell-cell and cell-matrix interactions in a variety of tissues. In particular, the protein was found to be expressed in glial cells of developing, but not adult, peripheral nerves, where it takes part in signaling mediated by ErbB class of receptors for neuregulins. Here, we demonstrate, using high resolution morphological methods, tissue fractionation and RT-PCR, that CD44 is strongly expressed in terminal Schwann cell (TSC) at the neuromuscular junction (NMJ) of the adult rat skeletal muscle. As CD44 is also expressed by Schwann cells of the non-myelinated Remak bundles of the proximal peripheral nerves, it appears to be a marker of non-myelinating Schwann cell subpopulation. The analysis of transgenic rats bearing a mutated superoxide-dismutase gene (SOD1(G93A)) causing familial amyotrophic lateral sclerosis (ALS) revealed that TSC activation and morphological plasticity at the NMJ, caused by ongoing denervation-reinnervation is associated with a strong increase in CD44 expression therein. Notably, CD44 immunoreactivity is present in fine axon-escheating processes of the glial cells that guide reinnervation. In addition, we found that both in normal and SOD1(G93A) muscle, CD44 expressed in TSC partially colocalizes with immunoreactivities of neuregulin receptors ErbB2 and ErbB3. The colocalization appears to reflect a physical interaction, as evidenced by co-immunoprecipitation and fluorescence resonance energy transfer (FRET) analysis between CD44 and ErbB3. Importantly, TSC activation upon ALS-like neurodegeneration results in significant increase in molecular proximity of CD44 and ErbB3, which may have an impact on glial plasticity at the NMJ.
Adam Gorlewicz; Jakub Wlodarczyk; Ewa Wilczek; Maciej Gawlak; Anna Cabaj; Henryk Majczynski; Klaudia Nestorowicz; Magdalena Aneta Herbik; Pawel Grieb; Urszula Slawinska; Leszek Kaczmarek; Grzegorz M Wilczynski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurobiology of disease     Volume:  34     ISSN:  1095-953X     ISO Abbreviation:  Neurobiol. Dis.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-08-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9500169     Medline TA:  Neurobiol Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-58     Citation Subset:  IM    
Nencki Institute of Experimental Biology, Poland.
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MeSH Terms
Age Factors
Aging / metabolism
Amyotrophic Lateral Sclerosis / immunology,  metabolism,  physiopathology
Antigens, CD44 / genetics,  metabolism*
Fluorescence Resonance Energy Transfer
Glycoproteins / analysis,  metabolism
Nerve Degeneration / immunology,  metabolism*,  physiopathology
Nerve Fibers, Unmyelinated / metabolism,  ultrastructure
Neuroglia / cytology,  metabolism*
Neuromuscular Junction / immunology,  metabolism*,  physiopathology
Neuronal Plasticity / physiology*
RNA, Messenger / analysis,  metabolism
Rats, Sprague-Dawley
Rats, Transgenic
Receptor, erbB-3 / analysis,  metabolism
Schwann Cells / cytology,  metabolism*
Superoxide Dismutase / genetics
Reg. No./Substance:
0/Antigens, CD44; 0/Erbb2 protein, rat; 0/Glycoproteins; 0/RNA, Messenger; EC 1.15.1.-/superoxide dismutase 1; EC Dismutase; EC, erbB-3

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