| CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. | |
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MedLine Citation:
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PMID: 8104771 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We evaluated a flow cytometric method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes. |
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Authors:
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J K Nicholson; B M Jones; M Hubbard |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytometry Volume: 14 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1993 |
Date Detail:
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Created Date: 1993-10-29 Completed Date: 1993-10-29 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 685-9 Citation Subset: IM; X |
Affiliation:
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Immunology Branch, Centers for Disease Control, Atlanta, Georgia 30333. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD3 / analysis Antigens, CD4 / analysis Antigens, CD45 / analysis Blood Cells / cytology*, immunology CD4-Positive T-Lymphocytes / cytology*, immunology Child Flow Cytometry / methods* Fluorescent Dyes* HIV Seropositivity Humans Quality Control |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD3; 0/Antigens, CD4; 0/Fluorescent Dyes; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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