| CD4 T-helper cell cytokine phenotypes and antibody response following tetanus toxoid booster immunization. | |
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MedLine Citation:
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PMID: 23318779 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Routine methods for enumerating antigen-specific T-helper cells may not identify low-frequency phenotypes such as Th2 cells. We compared methods of evaluating such responses to identify tetanus toxoid- (TT) specific Th1, Th2, Th17 and IL10(+) cells. Eight healthy subjects were given a TT booster vaccination. Blood was drawn before, 3, 7, 14, and 28 d after vaccination and peripheral blood mononuclear cells (PBMC) were cultured for 7 d with TT, negative control (diluent), and a positive control (Staphylococcus enterotoxin B [SEB]). Activation markers (CD25 and CD69) were measured after 44h (n=8), cytokines in supernatant after 3 and 7 d, and intracellular cytokine staining (ICS) of proliferated cells (identified by dye dilution) after 7d (n=6). Vaccination increased TT-specific expression of CD25 and CD69 on CD3(+)CD4(+) lymphocytes, and TT-specific proliferation at 7, 14 and 28 d post vaccination. Vaccination induced TT-specific Th1 (IFN-γ, TNF-α, and IL-2) Th2 (IL-13, IL-5, and IL-4), Th17 (IL-17A) and IL-10(+) cells as measured by ICS. TT-specific Th1 cells were the most abundant (12-15% of all TT-specific CD4(+) T-cells) while IL10(+) (1.8%) Th17 (1.1%) and Th2 cells (0.2-0.6%) were less abundant. TT-specific cytokine concentrations in PBMC supernatants followed the same pattern where a TT-specific IL-9 response was also seen. In conclusion, TT booster vaccination induced a broad T-helper cell response. This method of evaluating cytokine phenotypes may be useful in examining the impact of nutrition and environmental conditions on the plasticity of T-helper cell memory responses. |
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Authors:
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Kimberly A Livingston; Xiaowen Jiang; Charles B Stephensen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-11 |
Journal Detail:
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Title: Journal of immunological methods Volume: - ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Published by Elsevier B.V. |
Affiliation:
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USDA Western Human Nutrition Research Center, 430 West Health Sciences Drive, Univ. of Calif., Davis, CA. 95616. Electronic address: kiliving@calpoly.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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