Document Detail

CD4+ T cells responsive through the indirect pathway can mediate skin graft rejection in the absence of interferon-gamma.
MedLine Citation:
PMID:  10755571     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: T cells responding through the indirect pathway can induce allograft rejection, but mechanisms of rejection are not known. Interferon-y (IFN-gamma) may be an important mediator of rejection under these circumstances. METHODS: We transferred CD4+ T cells from IFN-gamma-deficient (IFN-gamma-/-) mice into SCID recipients of MHC II-deficient (MHC II-/-) skin grafts. Under these conditions, rejection can only occur via the indirect pathway and cannot be mediated by T-cell production of IFN-gamma. RESULTS: IFN-gamma-/- CD4+ T cells rejected MHC II -/- skin grafts. Flow cytometry revealed only CD4+ T cells in the recipients. Cytokine enzyme-linked immunosorbent spot assays confirmed only indirect recognition with an associated expansion of an alloreactive population of IL-2-, IL-4-, and IL-5-secreting T cells. CONCLUSION: CD4+ T cells recognizing alloantigens via the indirect pathway can mediate skin graft rejection in the absence of IFN-gamma.
A Valujskikh; P S Heeger
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Transplantation     Volume:  69     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-04-19     Completed Date:  2000-04-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1016-9     Citation Subset:  IM    
Department of Medicine, Case Western Reserve University, and the Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Ohio 44106, USA.
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MeSH Terms
CD4-Positive T-Lymphocytes / metabolism,  physiology*
Cytokines / metabolism
Graft Rejection / physiopathology*
Histocompatibility Antigens Class II / analysis
Interferon-gamma / deficiency
Mice, Inbred Strains
Mice, Mutant Strains
Skin Transplantation*
Grant Support
Reg. No./Substance:
0/Cytokines; 0/Histocompatibility Antigens Class II; 82115-62-6/Interferon-gamma

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