Document Detail


CD4+ T cell enumeration in HIV infection with limited resources.
MedLine Citation:
PMID:  10022387     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The incidence of human immunodeficiency virus (HIV) infection continues to increase in South Africa. Limited resources are available for diagnosis and management of the disease and the development of affordable strategies is required. Absolute CD4 counts are used locally predominantly to monitor disease progression and institute prophylaxis against opportunistic infections. A dramatic increase in demand for CD4 counts prompted an investigation for a more cost-effective flow cytometry method than those currently recommended by the Centers for Disease Control (CDC). CD4 counts generated by two different single tube methods using CD3/CD4/CD8 [1(3)] and CD4 [1(1)] antibodies, respectively, were compared to the CDC recommended 6 tube 2 colour panel [6(2)]. Whole blood analysis using the Coulter Multi-Q-Prep system and an Epics XL Flow Cytometer (Coulter, Hialeah, FL) was performed for each of the three methods. Random samples from HIV positive adult patients were compared. A mean difference in the absolute CD4 counts of less than 10x10(6)/l was generated by both of the alternative panels when compared with the 6(2) panel. The precision of the three methods is comparable. In reagents alone, the 1(3) and 1(1) methods represent a cost saving of 76% and 93%, respectively, over the 6(2) method. The 1(3) and 1(1) panels would permit more affordable CD4 counts to be determined by the gold standard methodology of flow cytometry with no clinically significant sacrifices in accuracy or precision.
Authors:
G G Sherman; J S Galpin; J M Patel; B V Mendelow; D K Glencross
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  222     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-02     Completed Date:  1999-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  209-17     Citation Subset:  IM; X    
Affiliation:
Department of Haematology, School of Pathology, South African Institute for Medical Research, Johannesburg. gayles@mail.saimr.wits.ac.za
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MeSH Terms
Descriptor/Qualifier:
Adult
CD4 Lymphocyte Count / methods*
CD4-Positive T-Lymphocytes / cytology,  immunology
Flow Cytometry / methods
HIV Infections / blood,  immunology*
Humans
Monitoring, Immunologic
T-Lymphocyte Subsets

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