| CD4(+), HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells. | |
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MedLine Citation:
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PMID: 10629086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The involvement of HLA-class I in target cell lysis by CD4(+) cytolytic T cells (CTL) has been a controversial issue. A CTL clone of CD4 phenotype was derived from the peripheral blood lymphocytes of a patient with primary melanoma. The CTL clone stably lysed the autologous primary melanoma cells for approximately 9 months in culture. Both the Valpha2/Vbeta8 T-cell receptor and CD4 were involved in CTL cytotoxicity. Of a large panel of allogeneic primary and metastatic melanoma or colorectal carcinoma cells, autologous and allogeneic Epstein-Barr virus-transformed B cells and autologous fibroblasts, only allogeneic metastatic melanoma cells matched with the autologous tumor cells for HLA-class I (B57[17]) were lysed and induced IFN-gamma secretion by the CTL clone. Lysis of the autologous tumor cells was significantly blocked by monoclonal antibody to HLA-B17. Importantly, allogeneic, HLA-class I- and class II-unmatched melanoma cells were lysed by the CTL only following transfection of the cells with B57[17] cDNA. Our results provide direct evidence for the involvement of both CD4 and HLA-class I in tumor cell lysis by CD4(+) CTL. |
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Authors:
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R Somasundaram; P Robbins; D Moonka; E Loh; F Marincola; A Patel; D Guerry; D Herlyn |
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Publication Detail:
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Type: Case Reports; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 85 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-02-01 Completed Date: 2000-02-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 253-9 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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The Wistar Institute, Philadelphia, PA 19104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antibodies, Monoclonal / immunology CD4-Positive T-Lymphocytes / immunology* Cell Division Cytokines / secretion Cytotoxicity, Immunologic DNA, Complementary / genetics HLA-B Antigens / genetics, immunology Histocompatibility Antigens Class I / immunology* Humans Male Melanoma / immunology* Receptors, Antigen, T-Cell / immunology Skin Neoplasms / immunology* T-Lymphocytes, Cytotoxic / immunology* Transfection Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA 25874-17/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cytokines; 0/DNA, Complementary; 0/HLA-B Antigens; 0/HLA-B57; 0/Histocompatibility Antigens Class I; 0/Receptors, Antigen, T-Cell |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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