Document Detail


CD4 and CD8 antigen coexpression: a flow cytometric study of peripheral blood, bone marrow, body fluid, and solid lymphoreticular specimens.
MedLine Citation:
PMID:  9140307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: CD4 and CD8 antigen coexpression occurs not only on blastic T-cell malignancies, but also on a small subset of mature lymphocytes. The aim of this study was to determine the prevalence of this population of cells and to identify features that can be used to differentiate them from T lymphoblasts. DESIGN: All specimens submitted to the clinical flow cytometry laboratory from August 1, 1994, through July 31, 1995, were analyzed for CD4 and CD8 coexpression. MAIN OUTCOME MEASURE: Percentage of lymphocytes coexpressing the CD4 and CD8 antigens. RESULTS: Four percent (22/526) of all specimens contained a population of CD4/CD8 coexpressing cells. Five cases represented CD4 and CD8 antigen expression on neoplastic cells. In 17 cases, the CD4/CD8 coexpressing cells appeared to represent a population of mature lymphocytes with a normal phenotype. The immature cells of T-cell acute lymphocytic leukemia and lymphoblastic lymphoma represented a dominant uniform population of cells demonstrating strong staining with both the CD4 and CD8 antigens. Cases containing a mature population of CD4/CD8 coexpressing cells were characterized by fewer coexpressing cells and variable expression of CD8. There were cases where distinction of this population of mature CD4/CD8 coexpressing lymphoid cells from a blastic malignancy was not possible using immunophenotyping alone. CONCLUSION: Correlation of clinical, morphologic, and immunophenotypic data is recommended to prevent the misdiagnosis of subtle involvement by a blastic T-cell malignancy.
Authors:
E H Nah; D E King; F E Craig
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of pathology & laboratory medicine     Volume:  121     ISSN:  0003-9985     ISO Abbreviation:  Arch. Pathol. Lab. Med.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-29     Completed Date:  1997-05-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7607091     Medline TA:  Arch Pathol Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Clinical Pathology, Chonnam University Hospital, Kwangju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD4 / biosynthesis*
Antigens, CD8 / biosynthesis*
Ascitic Fluid / immunology,  pathology
Body Fluids / immunology,  metabolism*
Bone Marrow / metabolism*,  pathology
Eyelids / immunology,  pathology
Flow Cytometry
Humans
Leukocytes, Mononuclear / metabolism*,  pathology
Lymphoid Tissue / metabolism*,  pathology
Lymphoma / immunology,  pathology
Multiple Myeloma / immunology,  pathology
Pleural Effusion / immunology,  pathology
Chemical
Reg. No./Substance:
0/Antigens, CD4; 0/Antigens, CD8

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