Document Detail


CD4+ CD25+ regulatory T cells in human pregnancy: development of a Treg-MLC-ELISPOT suppression assay and indications of paternal specific Tregs.
MedLine Citation:
PMID:  17229266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The current study was aimed at developing a one-way mixed leucocyte culture-enzyme-linked immunospot (MLC-ELISPOT) assay for the study of CD4(+) CD25(+) regulatory T (T(reg)) cells and applying this method in the study of antifetal immune reactions during human pregnancy. Twenty-one pregnant women and the corresponding fathers-to-be, and 10 non-pregnant control women and men, participated in the study. CD4(+) CD25(+) cells were isolated from peripheral blood mononuclear cells (PBMC) by immunomagnetic selection. Maternal/control PBMC were stimulated with paternal or unrelated PBMC in MLC. Secretion of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) from responder cells, with or without the presence of autologous T(reg) cells, was analysed by ELISPOT. PBMC from pregnant women showed increased secretion of IL-4 compared to controls. In pregnant and non-pregnant controls, T(reg) cells suppressed IFN-gamma reactivity against paternal and unrelated alloantigens. Interestingly, T(reg) cells suppressed IL-4 secretion against paternal but not unrelated alloantigens during pregnancy. We have successfully developed a model for studying T(reg) cells in antifetal cytokine reactions during pregnancy. Results indicate that T(reg) cells contribute to strict regulation of both T helper type 1-like and type 2-like antifetal immune reactions. Interestingly, T helper type 2-like cells specific to unrelated alloantigens are able to escape the suppression of T(reg) cells, which would allow for IL-4, alongside CD4(+) CD25(+) T(reg) cells, to control potentially detrimental IFN-gamma reactions during pregnancy.
Authors:
Jenny Mjösberg; Göran Berg; Jan Ernerudh; Christina Ekerfelt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-11
Journal Detail:
Title:  Immunology     Volume:  120     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-08     Completed Date:  2007-05-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  456-66     Citation Subset:  IM    
Affiliation:
Unit for Autoimmunity and Immunoregulation, Faculty of Health Sciences, Department of Molecular and Clinical Medicine, Linköping University, Linköping, Sweden. jenmj@imk.liu.se
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Enzyme-Linked Immunosorbent Assay / methods
Female
Fetus / immunology
Flow Cytometry / methods
Forkhead Transcription Factors / biosynthesis,  genetics
Gene Expression / immunology
Humans
Immune Tolerance
Immunomagnetic Separation / methods
Interferon-gamma / biosynthesis
Interleukin-2 Receptor alpha Subunit / analysis*
Isoantigens / immunology
Lymphocyte Activation / immunology
Lymphocyte Culture Test, Mixed
Male
Middle Aged
Pregnancy / immunology*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction / methods
T-Lymphocyte Subsets / immunology*
T-Lymphocytes, Regulatory / immunology*
Th2 Cells / immunology
Chemical
Reg. No./Substance:
0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/Interleukin-2 Receptor alpha Subunit; 0/Isoantigens; 0/RNA, Messenger; 82115-62-6/Interferon-gamma
Comments/Corrections

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