| CD36 is one of important receptors promoting renal tubular injury by advanced oxidation protein products. | |
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MedLine Citation:
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PMID: 18971214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chronic accumulation of plasma advanced oxidation protein products (AOPPs) promotes renal fibrosis. However, the mechanism at the cellular level has not been clarified. In the present study, endocytic assay of human proximal tubular cells (HK-2 cells) demonstrated that AOPPs-human serum albumin (HSA) (in vitro preparations of chloramine- modified HSA) were significantly endocytosed in a dose-dependent manner at a higher level than HSA. The expression of CD36, a transmembrane protein of the class B scavenger receptor, in HK-2 cells was confirmed in the immunoblot analysis. In a cellular assay using overexpressing human CD36 in Chinese hamster ovary (CHO) cells, AOPPs-HSA were significantly endocytosed by CD36-CHO cells but not by mock-CHO cells. Furthermore, the endocytic association and degradation of AOPPs-HSA by HK-2 cells was significantly inhibited by anti-CD36 antibody treatment, suggesting that CD36 is partly involved in the uptake of AOPPs-HSA by HK-2 cells. AOPPs-HSA upregulated the expression of CD36 in a dose-dependent manner. In addition, AOPPs-HSA upregulated the generation of intracellular reactive oxygen species and the secretion of transforming growth factor (TGF)-beta1 in HK-2 cells, whereas anti-CD36 antibody neutralizes the upregulation of TGF-beta1. These results suggest that AOPPs-HSA may cause renal tubular injury via the CD36 pathway. |
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Authors:
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Yasunori Iwao; Keisuke Nakajou; Ryoji Nagai; Kenichiro Kitamura; Makoto Anraku; Toru Maruyama; Masaki Otagiri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-29 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: 295 ISSN: 1931-857X ISO Abbreviation: Am. J. Physiol. Renal Physiol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-05 Completed Date: 2009-03-12 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: United States |
Other Details:
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Languages: eng Pagination: F1871-80 Citation Subset: IM |
Affiliation:
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Dept. of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto Univ. 5-1 Oe-honmachi, Kumamoto 862-0973, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD36 / physiology* CHO Cells Cricetinae Cricetulus Endocytosis / physiology Fibrosis Humans Kidney / pathology Kidney Tubules / injuries* Oxidation-Reduction Proteins / metabolism* Reactive Oxygen Species / metabolism Serum Albumin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Proteins; 0/Reactive Oxygen Species; 0/Serum Albumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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