| CD36 is important for chylomicron formation and secretion and may mediate cholesterol uptake in the proximal intestine. | |
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MedLine Citation:
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PMID: 17030189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Studies are aimed to determine the role of CD36 in intestinal lipid absorption. METHODS: Knock-out (KO) and wild-type (WT) lymph fistula mice were used to study fatty acids (FA) and cholesterol uptake, and chylomicron formation and secretion. Uptake of FA and cholesterol was studied by using sucrose polybehenate and fecal dual isotope methods, respectively. RESULTS: The CD36 KO exhibited significant accumulation of dietary cholesterol in the intestinal lumen at the end of 6-hour lipid infusion and significant reduction of dietary cholesterol transport into the lymph. Fecal dual isotope studies, however, did not show any significant difference in cholesterol uptake, suggesting that given sufficient time, the KO intestine could compensate for the reduced cholesterol uptake observed in the acute lymph fistula studies. Recovery of dietary FA in the intestinal lumen was comparable between WT and KO, consistent with the sucrose polybehenate study. However, the KO mice accumulated more, albeit not significantly, dietary triacylglycerols in the intestine, followed by a significant reduction in lymphatic transport. The ratio of intestinal dietary triacylglycerols to FA was not higher in WT than KO, arguing against impaired lipid esterification. It is rather a deficiency in the formation and secretion of chylomicrons, as supported by the significantly less apolipoprotein B-48 and the smaller, albeit not significantly, lipoprotein particles secreted into the lymph of the KO. CONCLUSIONS: CD36 may play an important role in chylomicron formation and secretion and may also facilitate cholesterol uptake in the proximal intestine. |
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Authors:
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Andromeda M Nauli; Fatiha Nassir; Shuqin Zheng; Qing Yang; Chun-Min Lo; Sarah B Vonlehmden; Dana Lee; Ronald J Jandacek; Nada A Abumrad; Patrick Tso |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-08-16 |
Journal Detail:
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Title: Gastroenterology Volume: 131 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-10 Completed Date: 2006-12-04 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 1197-207 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Genome Research Institute, University of Cincinnati College of Medicine, 2120 Galbraith Road, Cincinnati, OH 45237, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD36 / genetics, metabolism* Apolipoproteins / metabolism, secretion Carbon Radioisotopes / diagnostic use Cholesterol / pharmacokinetics* Chromatography, Thin Layer Chylomicrons / metabolism*, secretion* Dietary Fats / pharmacokinetics Duodenum / metabolism*, secretion Lipoproteins / chemistry, metabolism Lymphatic System / metabolism Male Mice Mice, Knockout Particle Size Sucrose / pharmacology Triolein / pharmacokinetics Tritium / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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DK 33301/DK/NIDDK NIH HHS; DK 56863/DK/NIDDK NIH HHS; DK 56910/DK/NIDDK NIH HHS; DK 59630/DK/NIDDK NIH HHS; DK 60022/DK/NIDDK NIH HHS; P01 DK056863-050001/DK/NIDDK NIH HHS; P01 DK056863-06A10001/DK/NIDDK NIH HHS; R01 DK033301-19/DK/NIDDK NIH HHS; R01 DK056910-05/DK/NIDDK NIH HHS; R01 DK060022-04/DK/NIDDK NIH HHS; U24 DK059630-05/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Apolipoproteins; 0/Carbon Radioisotopes; 0/Chylomicrons; 0/Dietary Fats; 0/Lipoproteins; 10028-17-8/Tritium; 122-32-7/Triolein; 57-50-1/Sucrose; 57-88-5/Cholesterol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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