Document Detail


CD36 gene deletion decreases oleoylethanolamide levels in small intestine of free-feeding mice.
MedLine Citation:
PMID:  19778614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oleoylethanolamide (OEA) is an endogenous lipid mediator that decreases food intake and enhances lipid catabolism. Dietary fat stimulates OEA mobilization in the proximal small intestine, through a mechanism that requires the participation of the membrane glycoprotein CD36 (fatty acid translocase, FAT). CD36 is highly expressed in small-intestinal enterocytes and is involved in fatty acid uptake and intracellular signaling. Here, we analyze the impact of genetic CD36 deletion on OEA production in various mouse tissues under free-feeding conditions and at different times of the light/dark cycle. CD36 ablation decreases OEA levels in jejunum and plasma during the dark phase, when mice consume most of their daily food. CD36 deletion is also associated with reduced OEA levels in kidney, but not in other tissues including duodenum, stomach, adrenals, white and brown fat, heart, liver, pancreas, skeletal muscle and brain. The results underscore the important role of CD36 in jejunal OEA production linked to feeding.
Authors:
Ana Guijarro; Jin Fu; Giuseppe Astarita; Daniele Piomelli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-22
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  61     ISSN:  1096-1186     ISO Abbreviation:  Pharmacol. Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-08     Revised Date:  2011-07-19    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
Affiliation:
Department of Pharmacology, University of California, Irvine, CA 92697-4625, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36 / deficiency*,  genetics
Brain / metabolism
Circadian Rhythm
Down-Regulation
Fatty Acids / metabolism
Feeding Behavior*
Gene Deletion
Jejunum / metabolism*
Kidney / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Oleic Acids / blood,  metabolism*
Grant Support
ID/Acronym/Agency:
DK073955/DK/NIDDK NIH HHS; R01 DK073955-02/DK/NIDDK NIH HHS; R01 DK073955-03/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Fatty Acids; 0/Oleic Acids; 0/oleoylethanolamide
Comments/Corrections

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