Document Detail


CD34 and factor-XIIIa immunoreactivity in dermatofibrosarcoma protuberans and dermatofibroma.
MedLine Citation:
PMID:  9129699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The histologic distinction between dermatofibrosarcoma protuberans (DFSP) and the fibrous type of benign fibrous histiocytoma (dermatofibroma, DF) may be extremely difficult. In this study, we evaluated the extent and pattern of immunoreactivity of both CD34 and factor XIIIa in a large number of DFSPs and DFs in order to determine the utility of these markers in their distinction. Using histologic criteria alone, the authors independently evaluated and agreed upon 30 cases of DF and 24 cases of DFSP, and a representative section was stained with antibodies to both factor XIIIa and CD34. Immunopositivity was evaluated semiquantitatively and assigned a score from 0 to 5. CD34 immunoreactivity was seen in 22 (92%) of 24 DFSPs (mean CD34 score, 4.60 +/- 0.3). Only 12 (40%) of 30 DFs showed CD34 immunopositivity (mean CD34 score, 0.6 +/- 0.1). Factor XIIIa was seen in 29 (97%) of 30 DFs (mean factor-XIIIa score, 4.1 +/- 0.3). In contrast, 18 (75%) of 24 DFSPs stained for factor XIIIa (mean factor-XIIIa score, 1.3 +/- 0.2), but in most of these cases the factor-XIIIa-positive cells were felt to be entrapped nonneoplastic dermal dendrocytes. Thus, an immunoprofile using antibodies to CD34 and factor XIIIa is capable of distinguishing between DFSP and the fibrous type of DF in the vast majority of cases, as long as there is recognition that there may be some CD34-positive cells in DFs, as well as some factor-XIIIa-positive cells in DFSPs.
Authors:
J R Goldblum; R J Tuthill
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American Journal of dermatopathology     Volume:  19     ISSN:  0193-1091     ISO Abbreviation:  Am J Dermatopathol     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-07-03     Completed Date:  1997-07-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7911005     Medline TA:  Am J Dermatopathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  147-53     Citation Subset:  IM    
Affiliation:
Department of Anatomic Pathology, Cleveland Clinic Foundation, OH 44195, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD34 / analysis*
Coloring Agents / diagnostic use
Dendritic Cells / pathology
Dermatofibrosarcoma / pathology*
Diagnosis, Differential
Histiocytoma, Benign Fibrous / pathology*
Humans
Skin / pathology
Skin Neoplasms / pathology*
Transglutaminases / analysis*
Tumor Markers, Biological / analysis
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Coloring Agents; 0/Tumor Markers, Biological; EC 2.3.2.13/Transglutaminases

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