Document Detail


CD28 and inducible costimulatory protein Src homology 2 binding domains show distinct regulation of phosphatidylinositol 3-kinase, Bcl-xL, and IL-2 expression in primary human CD4 T lymphocytes.
MedLine Citation:
PMID:  12816995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ligation of either CD28 or inducible costimulatory protein (ICOS) produces a second signal required for optimal T cell activation and proliferation. One prominent difference between ICOS- and CD28-costimulated T cells is the quantity of IL-2 produced. To understand why CD28 but not ICOS elicits major increases in IL-2 expression, we compared the abilities of these molecules to activate the signal transduction cascades implicated in the regulation of IL-2. Major differences were found in the regulation of phosphatidylinositol 3-kinase activity (PI3K) and c-jun N-terminal kinase. ICOS costimulation led to greatly augmented levels of PI3K activity compared with CD28 costimulation, whereas only CD28 costimulation activated c-jun N-terminal kinase. To examine how these differences in signal transduction affected IL-2 production, we transduced primary human CD4 T cells with a lentiviral vector that expressed the murine CD28 extracellular domain with a variety of human CD28 and ICOS cytoplasmic domain swap constructs. These domains were able to operate as discrete signaling units, suggesting that they can function independently. Our results show that even though the ICOS Src homology (SH) 2 binding domain strongly activated PI3K, it was unable to substitute for the CD28 SH2 binding domain to induce high levels of IL-2 and Bcl-x(L). Moreover, the CD28 SH2 binding domain alone was sufficient to mediate optimal levels of Bcl-x(L) induction, whereas the entire CD28 cytoplasmic tail was required for high levels of IL-2 expression. Thus, differences within their respective SH2 binding domains explain, at least in part, the distinct regulation of IL-2 and Bcl-x(L) expression following ICOS- or CD28-mediated costimulation.
Authors:
Richard V Parry; Catherine A Rumbley; Luk H Vandenberghe; Carl H June; James L Riley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  171     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-20     Completed Date:  2003-09-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  166-74     Citation Subset:  AIM; IM    
Affiliation:
Abramson Family Cancer Research Institute and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / biosynthesis*,  metabolism
Adjuvants, Immunologic / genetics,  metabolism,  physiology
Animals
Antigens, CD28 / genetics,  metabolism,  physiology*
Antigens, Differentiation, T-Lymphocyte / genetics,  metabolism,  physiology*
CD4-Positive T-Lymphocytes / enzymology*,  immunology*,  metabolism
Cell Line
Cytoplasm / genetics,  physiology
Enzyme Activation / immunology
Humans
Interleukin-2 / biosynthesis*,  metabolism
JNK Mitogen-Activated Protein Kinases
Membrane Proteins / genetics,  physiology
Mice
Mitogen-Activated Protein Kinases / metabolism
Models, Immunological
Peptide Fragments / genetics,  physiology
Protein Binding / genetics,  immunology
Proto-Oncogene Proteins c-bcl-2 / biosynthesis*,  metabolism
Recombinant Fusion Proteins / physiology
Signal Transduction / genetics,  immunology
bcl-X Protein
src Homology Domains / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Antigens, CD28; 0/Antigens, Differentiation, T-Lymphocyte; 0/BCL2L1 protein, human; 0/Bcl2l1 protein, mouse; 0/Interleukin-2; 0/Membrane Proteins; 0/Peptide Fragments; 0/Proto-Oncogene Proteins c-bcl-2; 0/Recombinant Fusion Proteins; 0/bcl-X Protein; 0/inducible T-cell co-stimulator; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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