| CD25, CD28 and CD38 expression in peripheral blood lymphocytes as a tool to predict acute rejection after liver transplantation. | |
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MedLine Citation:
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PMID: 18565100 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIM: The aim of this study was to determine whether the expression of CD25, CD28 and CD38 (which reflects the degree of T-cell activation) by peripheral blood mononuclear cells constitutes a useful means of measuring the immune status of liver transplant recipients. METHODS: Fifty-two patients enrolled in a prospective randomized study comparing cyclosporine and tacrolimus as the principal immunosuppressive drugs were monitored prospectively. The expression of CD25, CD28 and CD38 was analyzed on CD3-, CD4- and CD8-positive cells from whole blood using flow cytometry. The prognostic value of baseline and day 14 measurements regarding acute rejection was examined using Kaplan-Meier estimates for univariate analyses and the Cox model for multivariate analyses. RESULTS: The mean frequencies of CD28 and CD38-expressing T cells were significantly higher in patients with acute rejection (p = 0.01 and p = 0.001, respectively), whereas the frequency CD25-expressing T cells did not differ significantly. Under univariate analysis, baseline CD25 levels, the type of calcineurin inhibitor, as well as the CD28 and CD38 frequencies obtained at day 14 were associated with the subsequent development of acute rejection. Under multivariate analysis, only CD28 and CD38 frequencies obtained at day 14 were independently associated with acute rejection. CONCLUSIONS: The evaluation of CD28 and CD38 expression in peripheral blood lymphocytes is a simple marker that could be used routinely in clinical practice to assess the level of immunosuppression. |
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Authors:
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Emmanuel Boleslawski; Samia BenOthman; Sophie Grabar; Leonor Correia; Philippe Podevin; Sandrine Chouzenoux; Olivier Soubrane; Yvon Calmus; Filomena Conti |
Publication Detail:
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Type: Journal Article; Randomized Controlled Trial Date: 2008-06-28 |
Journal Detail:
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Title: Clinical transplantation Volume: 22 ISSN: 1399-0012 ISO Abbreviation: Clin Transplant Publication Date: 2008 Jul-Aug |
Date Detail:
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Created Date: 2008-08-27 Completed Date: 2008-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8710240 Medline TA: Clin Transplant Country: Denmark |
Other Details:
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Languages: eng Pagination: 494-501 Citation Subset: IM |
Affiliation:
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Laboratoire de Biologie Cellulaire, UPRES 1833, Faculté de Médecine Paris 5, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD28 / metabolism* Antigens, CD38 / metabolism* Cyclosporine / therapeutic use Female Flow Cytometry Graft Rejection / diagnosis*, immunology Humans Immunosuppressive Agents / therapeutic use Interleukin-2 Receptor alpha Subunit / metabolism* Liver Diseases / therapy* Liver Transplantation* Male Middle Aged Prognosis Prospective Studies T-Lymphocytes / immunology* Tacrolimus / therapeutic use Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD28; 0/Immunosuppressive Agents; 0/Interleukin-2 Receptor alpha Subunit; 109581-93-3/Tacrolimus; 59865-13-3/Cyclosporine; EC 3.2.2.5/Antigens, CD38 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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