Document Detail

CD147-targeting siRNA inhibits cell-matrix adhesion of human malignant melanoma cells by phosphorylating focal adhesion kinase.
MedLine Citation:
PMID:  21967109     Owner:  NLM     Status:  Publisher    
CD147/basigin, highly expressed on the surface of malignant tumor cells including malignant melanoma (MM) cells, plays a critical role in the invasiveness and metastasis of MM. Metastasis is an orchestrated process comprised of multiple steps including adhesion and invasion. Integrin, a major adhesion molecule, co-localizes with CD147/basigin on the cell surface. Using the human MM cell line A375 that highly expresses CD147/basigin, we investigated whether CD147/basigin is involved in adhesion in association with integrin. CD147/basigin was knocked-down using siRNA targeting CD147 to elucidate the role of CD147/basigin. Cell adhesion was evaluated by adhesion assay on matrix-coated plates. The localization of integrin was inspected under a confocal microscope and the expression and phosphorylation of focal adhesion kinase (FAK), a downstream kinase of integrin, were examined by western blot analysis. Silencing of CD147/basigin in A375 cells by siRNA induced the phosphorylation of FAK at Y397. Integrin identified on the surface of parental cells was distributed in a speckled fashion in the cytoplasm of CD147 knockdown cells, resulting in morphological changes from a round to a polygonal shape with pseudopodial protrusions. Silencing of CD147/basigin in A375 cells clearly weakened their adhesiveness to collagen I and IV. Our results suggest that CD147/basigin regulates the adhesion of MM cells to extracellular matrices and of integrin β1 signaling via the phosphorylation of FAK.
Rie Nishibaba; Yuko Higashi; Juan Su; Tatsuhiko Furukawa; Kazuhiro Kawai; Takuro Kanekura
Related Documents :
21956619 - Fitc labeled silica nanoparticles as efficient cell tags: uptake and photostability stu...
21902779 - Descemet membrane substrate from human donor lens anterior capsule.
22250009 - The effect of surface charge property on escherichia coli initial adhesion and subseque...
21643819 - Evolution of pva gels prepared without crosslinking agents as a cell adhesive surface.
21874229 - Simvastatin reduces tumor cell adhesion to human peritoneal mesothelial cells by decrea...
21744069 - Inhibition of helicobacter pylori adhesion to kato iii cells by intact and low molecula...
7930589 - The in vivo and in vitro effects of antibodies against rabbit beta 2-integrins.
16651639 - Differential regulation of pulmonary endothelial monolayer integrity by varying degrees...
20068579 - Vascular endothelial growth factor increases neurogenesis after traumatic brain injury.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-3
Journal Detail:
Title:  The Journal of dermatology     Volume:  -     ISSN:  1346-8138     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600545     Medline TA:  J Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Japanese Dermatological Association.
Departments of Dermatology Molecular Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan Department of Dermatology, XiangYa Hospital, Central South University, Changsha, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Proteomic Identification of Protease Cleavage Sites Characterizes Prime- and Non-prime Specificity o...
Next Document:  Infrared heat treatment reduces food intake and modifies expressions of TRPV3-POMC in the dorsal med...