Document Detail

CD14-dependent alterations in c-Jun expression in the liver after burn injury.
MedLine Citation:
PMID:  15522312     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Burn injury-triggered activation of lipopolysaccharide signaling via the CD14 pathway alters the expression of a variety of downstream genes contributing to pathogenic changes in distant organs. The regulation of CD14 and its role in the immediate-early response of c-Jun in the liver after burn injury were investigated in this study. MATERIALS AND METHODS: An incidental identification of the differential induction of CD14 mRNA after an approximately 18% TBSA burn injury in mice was confirmed by RT-PCR and immunohistochemical analyses of CD14 expression. Subsequently, CD14's role in the immediate-early regulation of c-Jun expression in the liver after injury was examined by Western blot analysis using CD14 knockout (KO) mice. RESULTS: RT-PCR analysis demonstrated a rapid and transient induction of CD14 mRNA in the liver and lungs of mice after injury. Immunohistochemical analysis revealed a peak induction of CD14 reactivity in cells appearing to be Kupffer cells at day 1 after injury. Furthermore, an augmented and delayed induction of c-Jun mRNA was observed in the liver of CD14 KO mice after injury compared to wild-type controls. The induction of phosphorylated (serine 63 or serine 73) forms of c-Jun after injury was lower in the livers of CD14 KO mice than that in WT controls. CONCLUSIONS: This study provides evidence that injury elicits CD14 induction as well as hyperphosphorylation of the c-Jun N-terminus activation domain and that CD14 is involved in the modulation of c-Jun's transactivation potential via phosphorylation, which may be associated with hepatic pathogenesis after injury.
Kiho Cho; Lee K Adamson; Jayoung Jeong; Sicily D Crivello; Tajia G Vanhook; Tina Palmieri; David G Greenhalgh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of surgical research     Volume:  122     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-03     Completed Date:  2004-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-42     Citation Subset:  IM    
Burn Research, Shriners Hospitals for Children Northern California, Sacramento, California, USA.
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MeSH Terms
Antigens, CD14 / genetics,  metabolism*
Burns / metabolism*
Kupffer Cells / metabolism
Liver / metabolism*
Lung / metabolism
Mice, Inbred C57BL
Mice, Knockout
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
RNA, Messenger / biosynthesis,  metabolism
Reg. No./Substance:
0/Antigens, CD14; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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