| CD137 ligand signaling induces human monocyte to dendritic cell differentiation. | |
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MedLine Citation:
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PMID: 20432236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ligand for CD137 (4-1BB) is expressed on peripheral human monocytes and delivers a potent activating signal via reverse signaling. Here we show that treatment of monocytes with a recombinant CD137 protein that induces reverse signaling through CD137 ligand reduces typical macrophage characteristics such as phagocytosis, oxidative burst and CD14 expression; however, typical DC characteristics including endocytosis, costimulatory molecule expression and the ability to stimulate proliferation of naïve T cells are induced. CD137-generated DC do not express DC-SIGN, CD1a or IL-12 and secrete less IL-10 than classical DC. CD137-generated DC are mature, and addition of LPS+IFN-gamma does not enhance their T-cell-stimulatory capacity. This indicates that CD137 as a sole factor is sufficient to induce development to mature DC, making stimulation of CD137 ligand the most simple protocol to generate mature DC. CD137-generated DC are more potent in inducing T-cell proliferation than classical DC. They inhibit development of Treg cells but induce T-cell expression of perforin, IFN-gamma, IL-13 and IL-17. These data demonstrate that CD137 as a single factor is sufficient to induce differentiation of peripheral monocytes to mature inflammatory DC that have a more potent T-cell-stimulatory capacity than classical DC. |
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Authors:
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Shaqireen Kwajah M M; Herbert Schwarz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of immunology Volume: 40 ISSN: 1521-4141 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-23 Completed Date: 2010-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: Germany |
Other Details:
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Languages: eng Pagination: 1938-49 Citation Subset: IM |
Affiliation:
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Department of Physiology and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD137
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pharmacology* Antigens, CD14 / biosynthesis Antigens, Differentiation / biosynthesis Cell Differentiation / drug effects Cell Proliferation / drug effects Cells, Cultured Cytokines / biosynthesis, genetics Dendritic Cells / drug effects*, metabolism, pathology Endocytosis / drug effects Humans Lymphocyte Activation / drug effects Monocytes / drug effects*, metabolism, pathology Oxidation-Reduction / drug effects Perforin / biosynthesis, genetics Phagocytosis / drug effects Recombinant Proteins / pharmacology* Signal Transduction / drug effects T-Lymphocytes / drug effects*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD137; 0/Antigens, CD14; 0/Antigens, Differentiation; 0/Cytokines; 0/Recombinant Proteins; 126465-35-8/Perforin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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