Document Detail

CD133+ cells from medulloblastoma and PNET cell lines are more resistant to cyclopamine inhibition of the sonic hedgehog signaling pathway than CD133- cells.
MedLine Citation:
PMID:  20480407     Owner:  NLM     Status:  MEDLINE    
CD133 has recently been used as a reliable marker for brain tumor stem cells isolation. Sonic hedgehog (SHH) is implicated in medulloblastoma and central primitive neuroectodermal tumor (cPNET) formation. It has recently been suggested a role for the EWS/FLI1 fusion protein--typical of pPNET--in the upregulation of GLI1 and PTCH1 genes. Cyclopamine inhibits the SHH pathway in medulloblastoma cell lines, but its effect on cPNET and pPNET cell lines has not been well established yet. Our purpose was to study the effect of cyclopamine on medulloblastoma and PNET cell lines and to analyze whether CD133 expression might be able to modify this effect. We analyzed gene expression, cell viability, apoptosis, and tumorigenic capability before and after cyclopamine treatment in CD133 high-expressing and CD133 low-expressing cell lines. All medulloblastoma and PNET cell lines displayed an inhibitory effect on the expression of SHH pathway genes, on viability, and on tumorigenic potential after treatment. Nevertheless, CD133 expression made the cells more resistant to cyclopamine inhibition. These results open new doors to the understanding of CD133+ cancer stem cells as residual cells that might be responsible for treatment resistance.
Mónica Enguita-Germán; Paula Schiapparelli; Juan A Rey; Javier S Castresana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-18
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  31     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  381-90     Citation Subset:  IM    
Brain Tumor Biology Unit, University of Navarra School of Sciences, Pamplona, Spain.
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MeSH Terms
Antigens, CD / metabolism*
Apoptosis / drug effects
Brain Neoplasms / metabolism
Cell Line, Tumor
Cell Separation
Cell Survival / drug effects
Cerebellar Neoplasms / metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene Expression / drug effects
Glycoproteins / metabolism*
Hedgehog Proteins / metabolism*
Medulloblastoma / metabolism*
Neuroectodermal Tumors, Primitive / metabolism*
Peptides / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / drug effects*
Veratrum Alkaloids / pharmacology*
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Hedgehog Proteins; 0/Peptides; 0/Veratrum Alkaloids; 4449-51-8/cyclopamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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