Document Detail

CD10 is expressed on human thymic epithelial cell lines and modulates thymopentin-induced cell proliferation.
MedLine Citation:
PMID:  9337153     Owner:  NLM     Status:  MEDLINE    
Thymic hormones such as thymopoietin (TP) have been shown to regulate thymocyte differentiation and lymphocyte activation. However, it is not known whether thymopoietin affects thymic epithelial cell (TEC) functions. In this study we have examined the effect of a five amino acid active peptide (TP5), corresponding to amino acids 32-36 of TP, on the proliferation of nontransformed clones of human TEC. Our results indicate that TP5 induced reinitiation of DNA synthesis and potentiated fetal calf serum (FCS)-induced cell growth in postnatal and fetal-derived human TEC. We also found that TEC lines express high levels of endopeptidase 24.11, a cell-surface metallopeptidase also known as the CD10 antigen. We show that TP5 is cleaved by CD10 at the surface of TEC lines, indicating that this endopeptidase may regulate TP5-induced TEC proliferation. Phosphoramidon, a specific endopeptidase 24.11 inhibitor, consistently acts in synergy with TP5 to enhance FCS-induced TEC growth. Hence, we conclude that 1) TP5 alone or in combination with FCS supports the growth of TEC lines, and 2) TEC lines express high levels of CD10, which regulates TP5-induced TEC proliferation by acting as a thymic peptide degrading enzyme.
S Guérin; B Mari; E Fernandez; N Belhacene; M L Toribio; P Auberger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  11     ISSN:  0892-6638     ISO Abbreviation:  FASEB J.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-05     Completed Date:  1997-11-05     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1003-11     Citation Subset:  IM    
CJF INSERM 96.05, Faculté de Médecine, Nice, France.
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MeSH Terms
Antigens, CD / biosynthesis*,  physiology
Cell Division / drug effects
Cell Line
DNA / biosynthesis
Epithelial Cells / cytology,  drug effects,  physiology*
Neprilysin / biosynthesis*,  physiology
Protease Inhibitors / pharmacology
Stromal Cells / cytology,  immunology
Thymopentin / pharmacology*
Thymus Gland / cytology*,  immunology,  physiology
Reg. No./Substance:
0/Antigens, CD; 0/Protease Inhibitors; 69558-55-0/Thymopentin; 9007-49-2/DNA; EC

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