Document Detail


CCT chaperonin complex is required for the biogenesis of functional Plk1.
MedLine Citation:
PMID:  15923617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experiments from several different organisms have demonstrated that polo-like kinases are involved in many aspects of mitosis and cytokinesis. Here, we provide evidence to show that Plk1 associates with chaperonin-containing TCP1 complex (CCT) both in vitro and in vivo. Silencing of CCT by use of RNA interference (RNAi) in mammalian cells inhibits cell proliferation, decreases cell viability, causes cell cycle arrest with 4N DNA content, and leads to apoptosis. Depletion of CCT in well-synchronized HeLa cells causes cell cycle arrest at G(2), as demonstrated by a low mitotic index and Cdc2 activity. Complete depletion of Plk1 in well-synchronized cells also leads to G(2) block, suggesting that misfolded Plk1 might be responsible for the failure of CCT-depleted cells to enter mitosis. Moreover, partial depletion of CCT or Plk1 leads to mitotic arrest. Finally, the CCT-depleted cells reenter the cell cycle upon reintroduction of the purified constitutively active form of Plk1, indicating that Plk1 might be a CCT substrate.
Authors:
Xiaoqi Liu; Chin-Yo Lin; Ming Lei; Shi Yan; Tianhua Zhou; Raymond L Erikson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  25     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-30     Completed Date:  2005-07-14     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4993-5010     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138, USA. liu13@fas.harvard.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Cell Cycle / physiology*
Cell Cycle Proteins / genetics,  metabolism*
Chaperonin Containing TCP-1
Chaperonins / genetics,  metabolism*
Hela Cells
Humans
Phenotype
Protein Kinases / genetics,  metabolism*
Protein-Serine-Threonine Kinases
Proto-Oncogene Proteins / genetics,  metabolism*
RNA, Small Interfering / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
GM59172/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Small Interfering; 0/Recombinant Fusion Proteins; 0/TCP1 protein, human; 156288-95-8/CDC20 protein, human; EC 2.7.-/Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1; EC 3.6.1.-/Chaperonin Containing TCP-1; EC 3.6.1.-/Chaperonins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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