Document Detail

CCS and SOD1 mRNA are reduced after copper supplementation in peripheral mononuclear cells of individuals with high serum ceruloplasmin concentration.
MedLine Citation:
PMID:  17683925     Owner:  NLM     Status:  MEDLINE    
The limits of copper homeostatic regulation in humans are not known, making it difficult to define the milder effects of early copper excess. Furthermore, a robust assay to facilitate the detection of early stages of copper excess is needed. To address these issues, we assessed changes in relative mRNA abundance of methallothionein 2A (MT2A), prion (PrP), amyloid precursor-like protein 2 (APLP2), Cu/Zn superoxide dismutase (SOD1) and its copper chaperone (CCS) in peripheral mononuclear cells (PMNCs) from healthy adults representing the 5% highest and lowest extremes in the distribution curve of serum ceruloplasmin (Cp) concentrations of 800 individuals. The intracellular Cu content was also determined. PMNCs were isolated from individuals before and after exposure to a single daily dose of 10 mg Cu (as CuSO(4)) for 2 months. Results showed that although there were fluctuations in serum Cp values of the samples assessed before copper exposure, no significant differences were observed in cell copper content or in the relative abundance of MT2A, PrP and APLP2 transcripts in PMNCs. Also, these values were not modified after copper supplementation. However, CCS and SOD1 mRNA levels were reduced in PMNCs after copper supplementation in the individuals with the high Cp values, suggesting that they should be further explored as biomarkers of moderate copper overload in humans.
Miriam Suazo; Felipe Olivares; Marco A Mendez; Rodrigo Pulgar; Joseph R Prohaska; Miguel Arredondo; Fernando Pizarro; Manuel Olivares; Magdalena Araya; Mauricio González
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-01
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  19     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-04     Completed Date:  2008-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
Laboratory of Micronutrients, University of Chile (INTA), Santiago, Chile.
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MeSH Terms
Amyloid beta-Protein Precursor / genetics,  metabolism
Ceruloplasmin / analysis*
Copper / administration & dosage*,  metabolism
Leukocytes, Mononuclear / metabolism
Metallothionein / genetics,  metabolism
Molecular Chaperones / genetics,  metabolism*
Nerve Tissue Proteins / genetics,  metabolism
Prions / genetics,  metabolism
RNA, Messenger / metabolism
Superoxide Dismutase / genetics,  metabolism*
Reg. No./Substance:
0/APLP2 protein, human; 0/Amyloid beta-Protein Precursor; 0/CCS protein, human; 0/MT2A protein, human; 0/Molecular Chaperones; 0/Nerve Tissue Proteins; 0/Prions; 0/RNA, Messenger; 7440-50-8/Copper; 9038-94-2/Metallothionein; EC 1.15.1.-/superoxide dismutase 1; EC Dismutase; EC

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