Document Detail


CCAAT displacement protein, a regulator of differentiation-specific gene expression, binds a negative regulatory element within the 5' end of the human papillomavirus type 6 long control region.
MedLine Citation:
PMID:  9032333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have reported previously that a 636-bp fragment spanning the 5' two-thirds of the human papillomavirus type 6 (HPV6)-W50 long control region (LCR) functions as a transcriptional silencer (A. Farr, S. Pattison, B.-S. Youn, and A. Roman, J. Gen. Virol. 76:827-835, 1995). We have utilized nested deletion analyses to implicate a 66-bp sequence which appears to be critical for this activity. A comparison of the transcriptional regulatory activities of the LCRs of HPV6-W50 and HPV6b (which has a 94-bp deletion, resulting in the elimination of the 66-bp sequence) indicates that sequences within the 94-bp region negatively regulate the activity of the intact HPV6 LCR. Two sequence-specific DNA-protein interactions were visualized via electrophoretic mobility shift assays. One of the binding events is mediated by the transcriptional repressor CCAAT displacement protein (CDP), a factor which is active in undifferentiated cells but inactive in terminally differentiated cells. This conclusion is based on the following three lines of evidence: (i) a consensus CDP binding site oligonucleotide serves as a competitor in band shift assays, (ii) the band shift complex is not seen when a CDP-negative nuclear extract is used, and (iii) anti-CDP antiserum specifically inhibits the binding. These studies identify a DNA-protein interaction occurring within the 5' end of the LCR which may be important in maintaining the tight link between keratinocyte differentiation and HPV gene expression.
Authors:
S Pattison; D G Skalnik; A Roman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  71     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-03-18     Completed Date:  1997-03-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2013-22     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202-5120, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Differentiation
DNA, Viral
Gene Expression Regulation, Viral
Hela Cells
Homeodomain Proteins
Humans
Molecular Sequence Data
Nuclear Proteins / genetics,  metabolism*
Papillomaviridae / genetics*
Regulatory Sequences, Nucleic Acid*
Repressor Proteins / genetics,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
AI31494/AI/NIAID NIH HHS; R29CA58947/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/CUX1 protein, human; 0/DNA, Viral; 0/Homeodomain Proteins; 0/Nuclear Proteins; 0/Repressor Proteins
Comments/Corrections

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