| CC chemokine receptor 4 (CCR4) in human allergen-induced late nasal responses. | |
| | |
MedLine Citation:
|
PMID: 20148806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: CC Chemokine receptor 4 (CCR4) is preferentially expressed on Th2 lymphocytes. CCR4-mediated inflammation may be important in the pathology of allergic rhinitis. Disruption of CCR4 - ligand interaction may abrogate allergen-induced inflammation. METHODS: Sixteen allergic rhinitics and six nonatopic individuals underwent both allergen and control (diluent) nasal challenges. Symptom scores and peak nasal inspiratory flow were recorded. Nasal biopsies were taken at 8 h post challenge. Sections were immunostained and examined by light or dual immunofluorescence microscopy for eosinophils, T-lymphocytes, CCR4(+)CD3(+) and CXCR3(+)CD3(+) cells and examined by in situ hybridization for CCR4, IL-4 and IFN-gamma mRNA(+) cells. Peripheral blood mononuclear cells were obtained from peripheral blood of nine normal donors and the CCR4(+)CD4(+) cells assessed for actin polymerization in response to the CCR4 ligand macrophage-derived chemokine (MDC/CCL22) and the influence of a CCR4 antagonist tested. RESULTS: Allergic rhinitics had increased early and late phase symptoms after allergen challenge compared to diluent; nonatopics did not respond to either challenge. Eosinophils, but not total numbers of CD3(+) T cells, were increased in rhinitics following allergen challenge. In rhinitics, there was an increase in CCR4(+)CD3(+) protein-positive cells relative to CXCR3(+)CD3(+) cells; CCR4 mRNA+ cells were increased and IL-4 increased to a greater extent than IFN-gamma. CCR4(+)CD4(+) T cells responded to MDC in vitro, and this response was inhibited by the selective CCR4 antagonist. CONCLUSION: Lymphocyte CCR4 expression is closely associated with induction of human allergen-induced late nasal responses. Blocking CCR4-ligand interaction may provide a novel therapeutic approach in allergic disease. |
| | |
Authors:
|
G Banfield; H Watanabe; G Scadding; M R Jacobson; S J Till; D A Hall; D S Robinson; C M Lloyd; K T Nouri-Aria; S R Durham |
Related Documents
:
|
15893986 - An immunohistochemical study of canine nasal aspergillosis. 19925886 - Peripheral th-17 cells in allergic rhinitis: new evidence. 18607886 - Superantigens and the expression of t-cell receptor repertoire in chronic rhinosinusiti... 19917276 - The putative lipid raft modulator miltefosine displays immunomodulatory action in t-cel... 10779756 - Antigen-experienced cd4 t cells display a reduced capacity for clonal expansion in vivo... 22033416 - Proteasome inhibitor lactacystin augments natural killer cell cytotoxicity of myeloma v... |
Publication Detail:
|
Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
|
Title: Allergy Volume: 65 ISSN: 1398-9995 ISO Abbreviation: Allergy Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-16 Completed Date: 2011-01-10 Revised Date: 2012-09-20 |
Medline Journal Info:
|
Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
|
Languages: eng Pagination: 1126-33 Citation Subset: IM |
Affiliation:
|
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College London, London, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Intranasal Adult Allergens / administration & dosage, immunology* Biopsy Female Humans Hypersensitivity, Immediate / immunology*, physiopathology Inflammation / immunology, physiopathology Male Nasal Mucosa / immunology, metabolism Receptors, CCR4 / genetics, metabolism* Rhinitis, Allergic, Perennial / immunology*, physiopathology Rhinitis, Allergic, Seasonal / immunology*, physiopathology Th2 Cells / immunology, metabolism* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
057704//Wellcome Trust; //Medical Research Council |
| Chemical | |
Reg. No./Substance:
|
0/Allergens; 0/Receptors, CCR4 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Towards a European registry of severe allergic reactions: current status of national registries and ...
Next Document: The Effect of High Gravidity on the Carcinogenesis of Mammary Gland in TA2 Mice.