| CBP and histone deacetylase inhibition enhance the transactivation potential of the HOXB7 homeodomain-containing protein. | |
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MedLine Citation:
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PMID: 10435624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Homeodomain-containing proteins are transcription factors regulating the coordinated expression of multiple target genes involved in development, differentiation and cellular transformation. In this study, we demonstrated that HOXB7, one member of this family, behaved as a transactivator in breast cancer cells. Deletion of either the HOXB7 N-terminal domain or the C-terminal acidic tail abolished this transcriptional effect, suggesting a combination of distinct functional transactivating domains. HOXB7 physically interacted both in vitro and in vivo with the coactivator CREB-binding protein (CBP). This interaction led to an enhanced transactivating potential and required the N-terminal of HOXB7 as well as two domains located at the C-terminal part of CBP. Moreover, trichostatin A, a deacetylase inhibitor, strongly enhanced the transcriptional properties of HOXB7. Our data therefore indicate that HOX proteins can directly interact with CBP and that acetylation/deacetylation may regulate their transcriptional properties. |
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Authors:
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A Chariot; C van Lint; M Chapelier; J Gielen; M P Merville; V Bours |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 18 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-08-17 Completed Date: 1999-08-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 4007-14 Citation Subset: IM |
Affiliation:
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Laboratory of Medical Chemistry and Medical Oncology, CHU B35, University of Liege Sart-Tilman, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms CREB-Binding Protein Cyclic AMP Response Element-Binding Protein / physiology* Drug Synergism Enzyme Inhibitors / pharmacology Histone Deacetylase Inhibitors* Histone Deacetylases / genetics, physiology* Homeodomain Proteins / genetics, physiology* Humans Nuclear Proteins / physiology* Peptide Fragments / genetics, physiology Plasmids / chemical synthesis Trans-Activators / genetics, physiology* Transcriptional Activation / physiology* Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/CREBBP protein, human; 0/Cyclic AMP Response Element-Binding Protein; 0/Enzyme Inhibitors; 0/HOXB7 protein, human; 0/Histone Deacetylase Inhibitors; 0/Homeodomain Proteins; 0/Nuclear Proteins; 0/Peptide Fragments; 0/Trans-Activators; EC 2.3.1.48/CREB-Binding Protein; EC 3.5.1.98/Histone Deacetylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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