Document Detail

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation.
MedLine Citation:
PMID:  23135344     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI.
METHODS: Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively.
RESULTS: Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal.
CONCLUSIONS: Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.
T Sivanarayana; Ch Ravi Krishna; G Jaya Prakash; K Murali Krishna; K Madan; B Sireesha Rani; G Sudhakar; G A Rama Raju
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Publication Detail:
Type:  Journal Article     Date:  2012-11-08
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  2013-07-12     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1327-34     Citation Subset:  IM    
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MeSH Terms
Antigens, Neoplasm / metabolism
Chromatin* / genetics,  ultrastructure
Chromomycin A3
DNA Fragmentation*
Embryonic Development
Infertility, Male* / genetics,  physiopathology
Middle Aged
Protamines / metabolism
Sperm Injections, Intracytoplasmic / methods
Spermatozoa* / abnormalities,  cytology
Reg. No./Substance:
0/Antigens, Neoplasm; 0/CASA antigen; 0/Chromatin; 0/Protamines; DVW027E7NL/Chromomycin A3

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