| CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation. | |
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MedLine Citation:
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PMID: 23135344 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. METHODS: Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. RESULTS: Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. CONCLUSIONS: Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome. |
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Authors:
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T Sivanarayana; Ch Ravi Krishna; G Jaya Prakash; K Murali Krishna; K Madan; B Sireesha Rani; G Sudhakar; G A Rama Raju |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-8 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: - ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Genetics Department, Krishna IVF Clinic, Krishna Nagar, Visakhapatnam, 530 002, Andhra Pradesh, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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