Document Detail

CART in the dorsal vagal complex: sources of immunoreactivity and effects on Fos expression and food intake.
MedLine Citation:
PMID:  12445972     Owner:  NLM     Status:  MEDLINE    
CART-peptide (CARTp) has been shown to suppress food intake, particularly when injected into the 4th ventricle of rats, and the presence of CART in nodose ganglia suggested a role in satiation. Based on retrograde tracing from the DVC combined with CART immunohistochemistry and supranodose vagotomy, we found that CART immunoreactivity in varicose fibers of the dorsal vagal complex originates from vagal afferents, sparse projections from the medullary reticular formation and the arcuate/retrochiasmatic nucleus of the hypothalamus, and most likely also from local CART neurons in the area postrema and NTS. In the nodose ganglia, 17% of neurons with projections to the stomach and 41% to the duodenum express CART-IR. CART-IR vagal afferents significantly contribute to the rich fiber plexus in mainly the commissural NTS and the adjacent area postrema. Injections of CARTp into the 4th ventricle strongly suppressed sucrose drinking and stimulated expression of c-Fos in the NTS. Injections of CARTp directly into various subnuclei of the NTS were less effective in suppressing food intake. The findings suggest that the critical site for CART's suppression of food intake is not in the termination zone of CART-containing vagal afferents in the commissural NTS, and that CART release from vagal afferent terminals plays a minor role in satiation. The functional role of CART in vagal afferents and the site of food intake suppression by 4th ventricular CARTp remain to be determined.
Huiyuan Zheng; Laurel M Patterson; Hans Rudolf Berthoud
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  957     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-26     Completed Date:  2003-02-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  298-310     Citation Subset:  IM    
Neurobiology of Nutrition Laboratory, Pennington Biomedical Research Center, Louisiana State University, 6400 Perkins Road, Baton Rouge, LA 70808, USA.
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MeSH Terms
Amygdala / cytology,  metabolism
Appetite Regulation / drug effects,  physiology*
Area Postrema / cytology,  drug effects,  metabolism*
Axons / drug effects,  metabolism,  ultrastructure
Duodenum / innervation,  physiology
Fourth Ventricle / drug effects,  physiology
Hypothalamus / cytology,  metabolism
Nerve Tissue Proteins / metabolism*,  pharmacology
Neurons, Afferent / cytology,  drug effects,  metabolism
Nodose Ganglion / cytology,  drug effects,  metabolism*
Proto-Oncogene Proteins c-fos / drug effects,  metabolism
Rats, Sprague-Dawley
Reticular Formation / cytology,  metabolism
Satiation / physiology
Solitary Nucleus / cytology,  drug effects,  metabolism*
Stomach / innervation,  physiology
Vagus Nerve / cytology,  drug effects,  metabolism*
Visceral Afferents / cytology,  drug effects,  metabolism*
Grant Support
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins c-fos; 0/cocaine- and amphetamine-regulated transcript protein

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