| CARD In Situ Hybridization: Sights and Signals. | |
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MedLine Citation:
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PMID: 12114699 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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During the last decade, several strategies have been developed to improve the detection sensitivity of in situ hybridization (ISV) by amplification of either target nucleic acid sequences prior to ISH (e.g., in situ PCRX or the detection signals after the hybridization procedures (signal amplification). Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, summarize applications as well as possible limitations of CARD 15K, and discuss some future directions of in situ nucleic acid detection using this amplification strategy. |
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Authors:
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Ernst J. M. Speel; Paul Komminoth |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Endocrine pathology Volume: 10 ISSN: 1559-0097 ISO Abbreviation: Endocr. Pathol. Publication Date: 1999 |
Date Detail:
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Created Date: 2002-Jul-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9009288 Medline TA: Endocr Pathol Country: - |
Other Details:
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Languages: ENG Pagination: 193-198 Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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