| CABYR RNAi plasmid construction and NF-κB signal transduction pathway. | |
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MedLine Citation:
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PMID: 20954286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To construct the CABYR RNAi plasmid and study its relation with the nuclear factor (NF)-κB signal transduction pathway. METHODS: Human CABYR mRNA sequence was obtained from GenBank. The structure of cDNA sequence for the short hairpin RNA was BbsI + sense + loop + antisense + transcription terminator + KpnI + BamHI. A CABYR silencing plasmid was constructed and transfected into the human embryo cell line 293T. Quantitative real-time polymerase chain reaction was used to analyze CABYR and NF-κB gene expression. RESULTS: The CABYR and NF-κB expressions were detected in 293T cells. The oligonucleotide (5'-GCTCAGATGTTAGGTAAAG-3') efficiently silenced the expression of CABYR. The expression of NF-κB was not significantly affected by silencing CABYR (P = 0.743). CONCLUSION: CABYR can be found in the human embryo cell line 293T. Cabyrmid 2 can efficiently silence its target, CABYR, indicating that CABYR is not related with the NF-κB signal transduction pathway. |
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Authors:
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Lin-Xiang Shi; Yao-Ming He; Lin Fang; Hong-Bo Meng; Li-Jun Zheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2011-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 4980-5 Citation Subset: IM |
Affiliation:
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Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China. ajijinwei@126.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Calcium-Binding Proteins / genetics, metabolism* Cell Line Humans I-kappa B Proteins / metabolism Molecular Sequence Data NF-kappa B / genetics, metabolism* Phosphoproteins / genetics, metabolism* Phosphorylation Plasmids RNA Interference* RNA, Messenger / metabolism Signal Transduction* Transfection |
| Chemical | |
Reg. No./Substance:
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0/CABYR protein, human; 0/Calcium-Binding Proteins; 0/I-kappa B Proteins; 0/NF-kappa B; 0/Phosphoproteins; 0/RNA, Messenger; 139874-52-5/NF-kappaB inhibitor alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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