Document Detail


CABYR RNAi plasmid construction and NF-κB signal transduction pathway.
MedLine Citation:
PMID:  20954286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To construct the CABYR RNAi plasmid and study its relation with the nuclear factor (NF)-κB signal transduction pathway.
METHODS: Human CABYR mRNA sequence was obtained from GenBank. The structure of cDNA sequence for the short hairpin RNA was BbsI + sense + loop + antisense + transcription terminator + KpnI + BamHI. A CABYR silencing plasmid was constructed and transfected into the human embryo cell line 293T. Quantitative real-time polymerase chain reaction was used to analyze CABYR and NF-κB gene expression.
RESULTS: The CABYR and NF-κB expressions were detected in 293T cells. The oligonucleotide (5'-GCTCAGATGTTAGGTAAAG-3') efficiently silenced the expression of CABYR. The expression of NF-κB was not significantly affected by silencing CABYR (P = 0.743).
CONCLUSION: CABYR can be found in the human embryo cell line 293T. Cabyrmid 2 can efficiently silence its target, CABYR, indicating that CABYR is not related with the NF-κB signal transduction pathway.
Authors:
Lin-Xiang Shi; Yao-Ming He; Lin Fang; Hong-Bo Meng; Li-Jun Zheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-02-01     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4980-5     Citation Subset:  IM    
Affiliation:
Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China. ajijinwei@126.com
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Calcium-Binding Proteins / genetics,  metabolism*
Cell Line
Humans
I-kappa B Proteins / metabolism
Molecular Sequence Data
NF-kappa B / genetics,  metabolism*
Phosphoproteins / genetics,  metabolism*
Phosphorylation
Plasmids
RNA Interference*
RNA, Messenger / metabolism
Signal Transduction*
Transfection
Chemical
Reg. No./Substance:
0/CABYR protein, human; 0/Calcium-Binding Proteins; 0/I-kappa B Proteins; 0/NF-kappa B; 0/Phosphoproteins; 0/RNA, Messenger; 139874-52-5/NF-kappaB inhibitor alpha
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