| C9orf100, a new member of the Dbl-family guanine nucleotide exchange factors, promotes cell proliferation and migration in hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 22327280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dbl-family guanine nucleotide exchange factors (GEFs) are important activators of Rho GTPases, which are significantly associated with tumorigenesis and metastasis. The catalytic ability of the Dbl-family GEFs to activate Rho GTPases depends on their Dbl-homology domain followed by a pleckstrin-homology domain. In the present study, we showed that C9orf100, a new member of the Dbl-family GEFs with a minimal catalytic unit, may contribute to hepatocellular carcinoma (HCC). Quantitative real-time PCR results demonstrated that C9orf100 was highly and widely upregulated in 42/44 (95.5%, >2‑fold) HCC specimens compared with adjacent non-cancerous livers, and this upregulation was correlated with intrahepatic metastasis and α-fetoprotein levels of HCC. Furthermore, the ectopic expression of C9orf100 promoted cell proliferation and colony formation in Huh-7 and YY-8103 cells, whereas silencing of C9orf100 resulted in the suppression of cell growth in MHCC-97H and PLC/PRF/5 cells. Flow cytometry confirmed this effect on MHCC-97H cell growth and indicated that C9orf100 may function in the G2/M phase. In addition, we showed that C9orf100 is involved in the positive regulation of HCC cell migration by a transwell chamber analysis. Our findings suggest that C9orf100 plays a potential oncogenic role in the development and metastasis of HCC. |
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Authors:
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Haixiao Wang; Yandong Li; Yuping Wang; Ze-Guang Han; Bing Cai |
Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2012-02-10 |
Journal Detail:
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Title: Molecular medicine reports Volume: 5 ISSN: 1791-3004 ISO Abbreviation: Mol Med Rep Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-03-12 Completed Date: 2012-07-03 Revised Date: 2013-02-22 |
Medline Journal Info:
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Nlm Unique ID: 101475259 Medline TA: Mol Med Rep Country: Greece |
Other Details:
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Languages: eng Pagination: 1169-74 Citation Subset: IM |
Affiliation:
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Department of Hepatobiliary Surgery, Wuxi People's Hospital of Nanjing Medical University, Jiangsu, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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genetics,
metabolism*,
pathology Cell Line, Tumor Cell Movement* Cell Proliferation* Female Guanine Nucleotide Exchange Factors / genetics, metabolism* Humans Liver Neoplasms / genetics, metabolism*, pathology Male Neoplasm Metastasis Neoplasm Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Guanine Nucleotide Exchange Factors; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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