Document Detail

C5a-induced in vitro basophil activation in patients with chronic urticaria: a pilot study.
MedLine Citation:
PMID:  19562298     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Complement component 5a (C5a) might be involved in the formation of wheals in patients with chronic urticaria (CU). We sought to compare the in vitro responsiveness of basophils to C5a in patients with CU and in a control group. METHODS: Basophil surface expression of activation marker CD63 induced by C5a, anti-FcepsilonRI mAb or anti-IgE pAb was measured using flow cytometry in 17 patients with CU and in 10 healthy controls. RESULTS: Patients with CU showed significantly greater basophil CD63 surface expression induced by C5a (median [interquartile range]; 16.4% [13-25.1]; P = 0.011) than the group of healthy controls (10.7% [7.2-16.8]). In contrast, basophil CD63 response to anti-IgE and anti-FcepsilonRI was lower in the CU group (12.3% [6-36.3]; 25.9% [12.5-60.5]) than in the control group (51.7% [6.7-84.3]; 62.1% [9.7-89.2]), although not statistically significant. CONCLUSION: Results of this pilot study suggest that patients with CU might have an enhanced basophil response to stimulation with C5a, indicating that further studies in CU basophil responsiveness are needed.
Peter Korosec; Tjasa Subic; Katja Adamic; Mira Silar; Mitja Kosnik
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wiener klinische Wochenschrift     Volume:  121     ISSN:  0043-5325     ISO Abbreviation:  Wien. Klin. Wochenschr.     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-29     Completed Date:  2009-09-28     Revised Date:  2010-10-20    
Medline Journal Info:
Nlm Unique ID:  21620870R     Medline TA:  Wien Klin Wochenschr     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  339-43     Citation Subset:  IM    
University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia.
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MeSH Terms
Antigens, CD / immunology*
Basophil Degranulation Test*
Basophils / immunology*
Chronic Disease
Complement C5a / immunology*
Flow Cytometry
Immunoglobulin E / immunology
Middle Aged
Pilot Projects
Platelet Membrane Glycoproteins / immunology*
Receptors, IgE / immunology
Urticaria / immunology*
Reg. No./Substance:
0/Antigens, CD; 0/CD63 antigen; 0/FcepsilonRI alpha-chain, human; 0/Platelet Membrane Glycoproteins; 0/Receptors, IgE; 37341-29-0/Immunoglobulin E; 80295-54-1/Complement C5a

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