Document Detail

C4d staining in renal allograft biopsies with early acute rejection and subsequent clinical outcome.
MedLine Citation:
PMID:  21527651     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES: Diffuse C4d staining in peritubular capillaries (PTCs) during an acute rejection episode (ARE) is the footprint of antibody-mediated rejection. In current clinical practice, diffuse C4d+ staining during acute rejection is regarded as an inferior prognostic sign. This case-control study investigated the prognostic role of mere C4d staining for graft outcome during an ARE in a well defined cohort of similarly ARE-treated patients.
DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: All kidney transplant recipients in the authors' center from January 1, 1995 to December 31, 2005 were reviewed. From these patients, 151 had a clinical ARE. Paraffin and/or frozen material was available for 128 patients showing a histologically proven ARE within the first 6 months after transplantation. All ARE patients were treated similarly with high-dose pulse steroids and in the case of steroid unresponsiveness with anti-thymocyte globulin. Biopsies were scored according to Banff criteria. Frozen and paraffin sections were stained by immunofluorescence (IF) and immunohistochemistry (IHC) for C4d, respectively, and scored for PTC positivity.
RESULTS: Diffuse C4d+ staining in PTCs was found in 12.5% and 4.2% sections stained by IF or by IHC, respectively. Four patients showed diffuse positive staining with both methods but showed no different risk profile from other patients. No relation between C4d staining and clinical parameters at baseline was found. C4d staining was not associated with steroid responsiveness, graft, or patient survival.
CONCLUSIONS: This study shows that C4d staining is not related to clinical outcome in this cohort of histologically proven early AREs.
Johanna M Botermans; Hanneke de Kort; Michael Eikmans; Klaas Koop; Hans J Baelde; Marko J K Mallat; Kim Zuidwijk; Cees van Kooten; Emile de Heer; Natascha N T Goemaere; Frans H J Claas; Jan A Bruijn; Johan W de Fijter; Ingeborg M Bajema; Marian C van Groningen
Publication Detail:
Type:  Journal Article     Date:  2011-04-28
Journal Detail:
Title:  Clinical journal of the American Society of Nephrology : CJASN     Volume:  6     ISSN:  1555-905X     ISO Abbreviation:  Clin J Am Soc Nephrol     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-10     Completed Date:  2011-09-01     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101271570     Medline TA:  Clin J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1207-13     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 by the American Society of Nephrology
Department of Pathology, Leiden University Medical Centre, Albinusdreef 2, L1-Q, Postbus 9600, 2300 RC Leiden, The Netherlands.
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MeSH Terms
Acute Disease
Capillaries / immunology,  metabolism,  pathology
Case-Control Studies
Complement C4 / immunology*,  metabolism*
Follow-Up Studies
Graft Rejection* / metabolism,  mortality,  pathology
Graft Survival / immunology*
Kidney Transplantation / immunology*,  mortality
Middle Aged
Predictive Value of Tests
Risk Factors
Staining and Labeling / methods,  statistics & numerical data
Transplantation, Homologous
Reg. No./Substance:
0/Complement C4

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